Materials and Reagents

1. 96-well Microplate Nunc maxisorp White (Thermo Fisher Scientific, Nunc, catalog number: 436110 )
   
2. Recombinant 6x HIS tagged protein
   
3. Streptavidin (New England Biolabs, catalog number: N7021S )
4. NaHCO₃
5. Phosphate buffered saline (PBS) (Life Technologies, Gibco^®, catalog number:  10010031 )
   
6. Tween 20
   
7. BSA
8. Biotinylated RNA
9. 1 M Tris (pH 7.5)
10. NaCl
11. Yeast tRNA (Life Technologies, Applied Biosystems^®, catalog number:  AM7119 )
    
12. 6x His mAb-HRP Conjugate (Clontech, catalog number: 631210 )
    
13. ECL Peroxidase Substrate Solution A and B (Pierce Antibodies, catalog number:  32106 )
14. Water used for all solution is RNAase free
15. q-PCR tape (R&D systems, catalog number: DY992 )
    
16. RiboLoc RNAase Inhibitor (Thermo Fisher Scientific, catalog number:  EO0381 )
17. RNA 3' End Biotinylation Kit (Pierce Antibodies, catalog number: 20160 ) 
    
18. Binding buffer (see Recipes)
19. PBS-T (see Recipes)
    
    

Equipment

1. Luminescence Plate reader (BMG LABTECH, FLUOstar OPTIMA)
2. Thermocycler
3. Centrifuges
   

Procedure