蛋白质-RNA酶联免疫吸附试验

Vanesa Olivares Illana; Robin  Farhaeus

doi:10.21769/BioProtoc.257

Improve Research Reproducibility A Bio-protocol resource

提交稿件
订阅
CN
- EN - English
- CN - 中文

Peer-reviewed

Protein-RNA ELISA Assay

蛋白质-RNA酶联免疫吸附试验

Vanesa Olivares Illana email

RF Robin Farhaeus email

发布: 2012年09月05日第2卷第17期 DOI: 10.21769/BioProtoc.257 浏览次数: 14858

下载 PDF

Q&A

引用

Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of Cancer Cell, featuring study using the protocol.

Jan 2012

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

相关实验方案

水稻幼苗RYMV接种和抗性评估方法

Agnès Pinel-Galzi [...] Laurence Albar

2018年06月05日 7235 阅读

生物材料中4-羟基壬烯醛与蛋白质加合物的高灵敏、可重复检测法

T. Blake Monroe and Ethan J. Anderson

2019年10月05日 3256 阅读

用于检测和定量人血浆或血清中抗病毒抗体的优化间接 ELISA 方案：以 SARS-CoV-2 为例的案例研究

Claire Baine [...] Jennifer Serwanga

2023年12月20日 2431 阅读

Abstract

Protein-RNA ELISA assay is an effective and quantitative method to study protein-RNA interactions in vitro. In this protocol we used recombinant 6x HIS tagged protein, but it works as well for non tagged proteins.

Keywords: RNA (RNA)

Protein (蛋白)

Interaction (相互作用)

RNA-ELISA (rna-elisa)

Materials and Reagents

96-well Microplate Nunc maxisorp White (Thermo Fisher Scientific, Nunc, catalog number: 436110 )
Recombinant 6x HIS tagged protein
Streptavidin (New England Biolabs, catalog number: N7021S )
NaHCO₃
Phosphate buffered saline (PBS) (Life Technologies, Gibco^®, catalog number: 10010031 )
Tween 20
BSA
Biotinylated RNA
1 M Tris (pH 7.5)
NaCl
Yeast tRNA (Life Technologies, Applied Biosystems^®, catalog number: AM7119 )
6x His mAb-HRP Conjugate (Clontech, catalog number: 631210 )
ECL Peroxidase Substrate Solution A and B (Pierce Antibodies, catalog number: 32106 )
Water used for all solution is RNAase free
q-PCR tape (R&D systems, catalog number: DY992 )
RiboLoc RNAase Inhibitor (Thermo Fisher Scientific, catalog number: EO0381 )
RNA 3' End Biotinylation Kit (Pierce Antibodies, catalog number: 20160 )
Binding buffer (see Recipes)
PBS-T (see Recipes)