多肽的合成

Improve Research Reproducibility A Bio-protocol resource

Peer-reviewed

Peptide Synthesis

PDF

Q&A

引用

Cited by

参见作者原研究论文

The authors used this protocol in:

Nov 2011

实验方案合集

相关实验方案

细菌膜蛋白在蛋白质脂质体中的体外整合

Hanako Nishikawa [...] Ken-ichi Nishiyama

2020年05月20日 6096 阅读

一种集成引物池合成技术和切口诱变的用户自定义突变方法

Paul J. Steiner [...] Timothy A. Whitehead

2020年08月05日 7663 阅读

利用液–液相分离构建人工金属酶反应微环境

Kaixin Wang [...] Tong Wu

2025年10月05日 2052 阅读

Abstract

This protocol describes the synthesis of peptides for affinity testing and bioconjugate with solid phase peptide synthesizer at a small scale.

Materials and Reagents

Dichloromethane (DCM)
Dimetylformamide (DMF) (Fisher Science, catalog number: D119-4 )
Trifluoroacetic acid (TFA) (ACRO, catalog number: 13972 )
Thioanisole (TIS) (Sigma-Aldrich, 233781 )
Ethanedithiol (EDT) (TCI, catalog number: E0032 )
2-(1H-Benzotriazol-1-yl)-1,1,3,3-tertramethyluronumhexafluorophosphate (HBTU) (Anaspec, catalog number: AS-21001 )
N,N-diisopropylethylamine (DIEA) (Santa Cruz Biotech, catalog number: sc-215490 )
Piperidine (biotech. grade, ≥99.5%) (Sigma-Aldrich, catalog number: 571261 )
Dithiothriotol (DTT)
Pyridine
Potassium cyanide
Ninhydrin
Fmoc protected amino acids:
Fmoc-Ile-OH, Fmoc-Glu (OtBu)-OH, Fmoc-Met-OH, Fmoc-Leu-OH, Fmoc-Lys (Boc)-OH, Fmoc-Arg (Pbf)-OH, Fmoc-Asn (Trt)-OH, Fmoc-Ser (tBu)-OH, Fmoc-Ala-OH, Fmoc-His (Trt)-OH, Fmoc-Gln (Trt)-OH, Fmoc-Val-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Fmoc-Pro-OH and Fmoc-Asp (OtBu)-OH, Fmoc-Gly-OH, Fmoc-Cys(Trt)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Trp(Boc)-OH.
Rink Amide Resin (0.66 mmol/g) (AAPPTec, catalog number: RRZ001 or equivalent)
C-18 column (Vydac, catalog number: 218TP510 or equivalent)
All reagents are ACS grade or synthesis grade
Anhydrous diethyl ether