小鼠全乳腺移植方案

Hayley Hansford; Yan Hong; Frances Kittrell; Daniel Medina; Fariba Behbod

doi:10.21769/BioProtoc.2326

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Peer-reviewed

Whole Mammary Gland Transplantation in Mice Protocol

小鼠全乳腺移植方案

Fariba Behbod email

发布: 2017年06月05日第7卷第11期 DOI: 10.21769/BioProtoc.2326 浏览次数: 8391

评审: Anonymous reviewer(s)

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参见作者原研究论文

The authors used this protocol in:

Cover of Journal of Cell Science, featuring study using the protocol.

14-Oct 2013

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

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Abstract

Whole Mammary Gland Transplantation involves transplanting an excised mammary gland into another, more suitable host. This method can be used to extend the life of a mammary gland past the mouse’s life span by transplanting the mammary gland of an older mouse into a young healthy mouse. As you can see in the video below (Video 1), by attaching it to the abdomen of the mouse, the gland will receive a steady blood supply and both epithelial and stromal cells will remain viable for up to one year. Although this method is not used often, it has been part of several experiments including determining whether the stroma or epithelium is the primary target in chemically induced mouse mammary tumorigenesis (Medina and Kittrell, 2005). To monitor transplants, palpate every week for tumor formation. The transplanted mammary gland may also be passaged serially every 8-10 weeks. Keep transplanted gland in the same mouse for no longer than one year.

Video 1. Whole mammary gland transplantation

Keywords: Mammary gland (乳腺)

Transplant (移植)

Viable epithelial cells (存活上皮细胞)

Mammary tumorigenesis (乳腺肿瘤发生)

Materials and Reagents

Surgical gloves (Cardinal Health, catalog number: 2D72N80X )
1 ml Tuberculin (TB) syringe (BD, catalog number: 309625 )
Dissolvable suture, size: 0000
Animals: 8-12-week old female mice
Note: Excised gland will need to be transplanted into the same strain of mouse. If donor and recipient are of different strains, the recipient must be immunocompromised (i.e., gland excised from Balb/c mouse and transplanted to NSG mouse).
Nembutal sodium solution CII (Pentobarbital sodium injection, USP) (Akorn, Oak Pharmaceuticals, NDC 76478-501-20 )
Ketofen^® (Ketoprofen) (Zoetis, catalog number: 10004029 )
70% ethanol

Equipment

Balance
Hair clipper (Remington, model: PG525 )
Hemostatic forceps (Roboz Surgical Instruments, catalog number: RS-7131L )
Sharp/Ball Tip scissors (Fine Science Tool, catalog number: 14086-09 )
Rat tooth forceps (Sklar Surgical Instruments, catalog number: 19-1260 )
Curved forceps (Fine Science Tool, catalog number: 11052-10 )
Heating pad (Sunbeam Products, catalog number: 000771-810-000U )
Wound Clip applier, remover and clips (BD, catalog number: 427638 )

Procedure

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如何引用

Hansford, H., Hong, Y., Kittrell, F., Medina, D. and Behbod, F. (2017). Whole Mammary Gland Transplantation in Mice Protocol. Bio-protocol 7(11): e2326. DOI: 10.21769/BioProtoc.2326.