Primary Tumor Preparation
原代肿瘤细胞制备
发布: 2012年06月20日第2卷第12期 DOI: 10.21769/BioProtoc.229 浏览次数: 14326
PDF 
Q&A
引用
收藏
Cited by
Abstract
This protocol has been developed for culturing primary glioblastoma cells. We have most experience in using it on rodent preparations, but it can also be used in culturing cells from other species.
Materials and Reagents
- Phosphate buffered saline (PBS)
- Fetal bovine serum (FBS)
- Trypsin (Corning, Cellgro®, catalog number: 25-054-CI )
- Minimum essential medium (MEM)
- HEPES
- Sucrose
- B104 conditioned media
- N2 supplement (Life Technologies, Invitrogen™, catalog number: 17502-048 )
- T3 (Sigma-Aldrich, catalog number: T2877 )
- Penicillin/streptomycin/amphotericin (Life Technologies, Invitrogen™, catalog number: 15240-062 )
- DMEM (Life Technologies, Invitrogen™, catalog number: 11965-167 )
- Poly-L-lysine
- PDGF-AA (Sigma-Aldrich)
- FGFb (Life Technologies, Gibco®)
- Basal media (in DMEM) (see Recipes)
Equipment
- Mesh (BD Biosciences, Falcon®)
- Shaking bath
- Centrifuges
- 6-well tissue culture plates
Procedure
English
中文翻译
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Lei, L. (2012). Primary Tumor Preparation. Bio-protocol 2(12): e229. DOI: 10.21769/BioProtoc.229.
Download Citation in RIS Format
分类
癌症生物学 > 通用技术 > 细胞生物学试验 > 细胞分离和培养
细胞生物学 > 细胞分离和培养 > 细胞生长
您对这篇实验方法有问题吗？
在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。
0/150
提问指南
+ 问题描述
写下详细的问题描述，包括所有有助于他人回答您问题的信息（例如实验过程、条件和相关图像等）。
发布问题0 Q&A
