Generation of Mouse Bone Marrow-Derived Dendritic Cells (BM-DCs)
培养老鼠骨髓树状细胞
发布: 2012年06月20日第2卷第12期 DOI: 10.21769/BioProtoc.226 浏览次数: 20408
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参见作者原研究论文
The authors used this protocol in:
Jul 2009
Abstract
Generating mouse dendritic cells from bone-marrow progenitor cells is a useful tool to study biological functions of mouse dendritic cells. Dendritic cells are one of the major populations of phagocytes able to activate both innate and adaptive immune cells.
Keywords: Phagocytes (吞噬细胞)
Dendritic cells (树突状细胞)
In vitro (体外)
GM-CSF (GM-CSF)
Materials and Reagents
- GM-CSF-transduced B16 cell line
- HI FBS (EuroClone, catalog number: EC S0180L )
- L-Glutamine (EuroClone, catalog number: EC B3000D )
- Penicillin/streptomycin (EuroClone, catalog number: EC B3001D )
- IMDM (EuroClone, catalog number: EC B2072L )
- Beta-mercaptoethanol (Sigma-Aldrich, catalog number: M6250 )
- B16-GMCSF growth supernatant
- Phosphate buffered saline (PBS) (EuroClone, catalog number: ECM9605AX )
- EDTA
- BMDCs culture medium/conditioned medium (see Recipes)
Equipment
- Centrifuges 70 μm-wide cut-off cell strainer
- Non-treated cell culture plates
- Incubator (37 °C and 5% CO2)
- Fluorescence activated cell sortor (FACS)
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Granucci, F., Ostuni, R. and Zanoni, I. (2012). Generation of Mouse Bone Marrow-Derived Dendritic Cells (BM-DCs). Bio-protocol 2(12): e226. DOI: 10.21769/BioProtoc.226.
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分类
免疫学 > 免疫细胞分离 > 维持和分化
细胞生物学 > 细胞分离和培养 > 细胞分化
干细胞 > 成体干细胞 > 维持和分化
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