Measurement of Liver Triglyceride Content
肝脏三磷酸酯含量的测定
发布: 2012年07月05日第2卷第13期 DOI: 10.21769/BioProtoc.223 浏览次数: 33917
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Abstract
This assay is designed to measure relative lipid accumulation of experimental treatments compared to controls. The reagent measures the concentration of glycerol released after lysing the cells and hydrolyzing the triglyceride molecules. The triglyceride concentration can then be determined from the glycerol values.
Background
Materials and Reagents
- EtOH
- KOH
- MgCl2
- Triglyceride (GPO Trinder) reagent A (Sigma-Aldrich, catalog number: 337-40A )
[Please note that this product has been replaced by free glycerol reagent (F6428) from Sigma]
- Glycerol Standards (Sigma-Aldrich, catalog number: 339-11 or G7793 )
- Ethanolic KOH (see Recipes)
Equipment
- Microfuge tube
- Glycerol
- Microreader
- Cuvette
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Jouihan, H. (2012). Measurement of Liver Triglyceride Content. Bio-protocol 2(13): e223. DOI: 10.21769/BioProtoc.223.
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分类
生物化学 > 脂质 > 脂质测定
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