Iron - Prussian Blue Reaction - Mallory’s Method
铁-普鲁士兰反应法- Mallory’s 方法
发布: 2012年07月05日第2卷第13期 DOI: 10.21769/BioProtoc.222 浏览次数: 27111
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Abstract
Purpose: To demonstrate ferric iron in tissue sections. Small amounts of iron are found normally in spleen and bone marrow. Excessive amounts are present in hemochromatosis, with deposits found in the liver and pancreas, hemosiderosis, with deposits in the liver, spleen, and lymph nodes.
Principle: The reaction occurs with the treatment of sections in acid solutions of ferrocyanides. Any ferric ion (+3) in the tissue combines with the ferrocyanide and results in the formation of a bright blue pigment called 'Prussian blue" or ferric ferrocyanide.
Materials and Reagents
- Control: A known positive control tissue (such as spleen)
- Formalin
- EtOH
- Hydrochloric acid
- Aluminum sulfate
- Histoclear reagent
- Mounting solution
- Fixative (see Recipes)
- 5 % potassium Ferrocyanide (see Recipes)
- 5% hydrochloric acid (see Recipes)
- Nuclear-fast red (Kernechtrot) (see Recipes)
- Working solution (see Recipes)
Equipment
- Microwave oven
- Acid-cleaned glassware
- Non-metallic forceps.
- Gloves, goggles and lab coat
- Fume hood
Procedure
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版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Jouihan, H. (2012). Iron - Prussian Blue Reaction - Mallory’s Method. Bio-protocol 2(13): e222. DOI: 10.21769/BioProtoc.222.
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分类
细胞生物学 > 组织分析 > 组织染色
细胞生物学 > 细胞染色 > 铁
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