Peer-reviewed
Soft–Agar colony Formation Assay
软琼脂克隆形成实验
发布: 2012年07月05日第2卷第13期 DOI: 10.21769/BioProtoc.220 浏览次数: 65837
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Abstract
Any anchorage–independent growth of tumor cells is estimated by a soft–agar colony formation assay. This protocol provides a general workflow for establishing a soft-agar colony formation assay.
Materials and Reagents
- Agarose-LE (Guidechem, catalog number: 9012-36-6 )
- DMEM (Life Technologies, Gibco®, catalog number: 11995 )
- Crystal violet (Matheson Coleman & BEL: catalog number: B278 )
- Fetal bovine serum (FBS) (Life Technologies, Invitrogen™, catalog number: 20140-079 )
- Phosphate buffered saline (PBS) (Life Technologies, Gibco®, catalog number: 14040 )
- Agar
- Ethanol
- Culture media (see Recipes)
Equipment
- 60 mm culture dishes (Thermo Fisher Scientific, catalog number: 353002 )
- Water bath
- Incubator
Procedure
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English
中文翻译
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Liu, F. (2012). Soft–Agar colony Formation Assay. Bio-protocol 2(13): e220. DOI: 10.21769/BioProtoc.220.
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分类
癌症生物学 > 通用技术 > 细胞生物学试验 > 细胞分离和培养
细胞生物学 > 细胞分离和培养 > 细胞生长
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