通过嫁接建立新的分根系统

Xiangqiang  Kong; Zhen  Luo; Hezhong  Dong

doi:10.21769/BioProtoc.2136

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Peer-reviewed

Establishment of New Split-root System by Grafting

通过嫁接建立新的分根系统

Xiangqiang Kong

ZL Zhen Luo

HD Hezhong Dong email

发布: 2017年02月20日第7卷第4期 DOI: 10.21769/BioProtoc.2136 浏览次数: 10592

评审: Marisa RosaTzvetina BrumbarovaAnonymous reviewer(s)

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参见作者原研究论文

The authors used this protocol in:

Cover of Journal of Experimental Botany, featuring study using the protocol.

Mar 2012

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Abstract

A new split-root system was used to simulate non-uniform salt, drought or nutrient deficiency stress in the root zone, in which the root system was divided into two or more equal portions. Here, we established a split-root system by grafting of cotton seedlings. In contrast to the conventional split-root, the main roots of the new system remained intact, which provided a better system for studying cotton response to unequal treatment in the root zone. The new system was suitable for plant growth in nutrient solution and the two root systems can fully be immerged in the nutrient solution.

Keywords: Cotton (棉花)

Split-root system (分根系统)

Graft (嫁接)

Unequal treatment (差异处理)

Scion (幼枝)

Stock (砧木)

Background

The split-root system has been used to study plant responses to heterogeneous soil conditions such as partial root drying, unequal salt distribution, and heterogeneous nutrient distribution. The conventional split-root system in cotton and other plants are established by dividing the lateral roots into two equal parts after cutting of the main root of a seedling (Bazihizina et al., 2009; Dong et al., 2010). The new system was suitable for plant growth in nutrient solution and for a girdling experiment because there was sufficient distance between the root and position of the graft (Kong et al., 2012 and 2016).

Materials and Reagents

Plastic boxes (60 x 45 x 15 cm) (Linhui, catalog number: LH-600 )
Sterilized wet sand
Blade (Pao Shen Enterprises, KW-trio^®, catalog number: 03541 )
Parafilm (Bemis, catalog number: PM996 )
Disposable cup (Jiaxing, catalog number: hot paper cup-HC02W )
Plastic bags (Yiwu, catalog number: 10*15 )
Cotton seeds (SCRC41, a commercial Bt [Bacillus thuringiensis] transgenic cotton [Gossypium hirsutum L.] which developed by the Cotton Research Center, Shandong Academy of Agricultural Sciences, Jinan)
Sulfuric acid, H₂SO₄ (Sinopharm Chemical Reagent, catalog number: 7664-93-9 )
Calcium nitrate, Ca(NO₃)₂ (Sinopharm Chemical Reagent, catalog number: 10124-37-5 )
Potassium nitrate, KNO₃ (Sinopharm Chemical Reagent, catalog number: 7757-79-1 )
Magnesium sulfate, MgSO₄ (Sinopharm Chemical Reagent, catalog number: 7487-88-9 )
Ammonium dihydrogen phosphate, NH₄H₂PO₄ (Sinopharm Chemical Reagent, catalog number: 7722-76-1 )
EDTA·FeNa (Sinopharm Chemical Reagent, catalog number: 15708-41-5 )
Orthoboric acid, H₃BO₃ (Sinopharm Chemical Reagent, catalog number: 10043-35-3 )
Zinc sulfate, ZnSO₄ (Sinopharm Chemical Reagent, catalog number: 7446-20-0 )
Copper sulfate, CuSO₄ (Sinopharm Chemical Reagent, catalog number: 7758-99-8 )
Manganese sulfate, MnSO₄ (Sinopharm Chemical Reagent, catalog number: 15244-36-7 )
(NH₄)₆Mo₇O₂₄ (Sinopharm Chemical Reagent, catalog number: 12027-67-7 )
Potassium hydroxide, KOH (Sinopharm Chemical Reagent, catalog number: 1310-58-3 )
Nutrient solution (see Recipes)