Immunofluorescence Analysis of Yeast Protein

Materials and Reagents

1. Yeast cells
   
2. Potassium phosphate monobasic (KH₂PO₄) (Sigma-Aldrich, catalog number: P8416 )
   
3. Potassium phosphate dibasic (K₂HPO₄·3H₂O) (Sigma-Aldrich, catalog number: P9666 )
   
4. Sorbitol (C₆H₁₄O₆) (Sigma-Aldrich, catalog number: S1876 )
   
5. BSA (Albumin from bovine serum) (Sigma-Aldrich, catalog number: A4503 )
   
6. Potassium chloride (KCl) (Thermo Fisher Scientific, catalog number: BP366-1 )
   
7. 37% formaldehyde solution (Thermo Fisher Scientific, catalog number: F75P1GAL )
   
8. Zymolyase (USB, catalog number: Z1001 )
   
9. Vectorshield
   
10. Poly-L-lysine
    
11. DAPI
    
12. Cytoseal 60
    
13. Phosphate buffer (see Recipes)
    
14. Sorbitol buffer (see Recipes)
    
15. Blocking buffer (see Recipes)
    

Equipment

1. Centrifuges
   
2. Shaker
   
3. Conical tube
   
4. Fluorescence microscope
   
5. 15 ml conical tube
   
6. Light microscope
   
7. Heat blot
   

Procedure