Materials and Reagents

1. 50 ml conical tubes (MIDSCI, catalog number: C50B )
   
2. 2 ml microfuge tubes (MIDSCI, catalog number: AVX-T-20-C )
   
3. Arabidopsis inflorescence tissue
   
4. Liquid nitrogen
   
5. Ethanol, pure (Sigma-Aldrich, catalog number: E7023 )
   
6. Acetone (Sigma-Aldrich, catalog number: 320110 )
   
7. 1 N NaOH (Sigma-Aldrich, catalog number: S8045 )
   
8. 3,4,5-trimethoxycinnamic acid (Sigma-Aldrich, catalog number: T70408 )
   
9. HCl (concentrated hydrochloric acid) (36-38%) (Sigma-Aldrich, catalog number: H1758 )
   
10. Ethyl acetate (Sigma-Aldrich, catalog number: 270989 )
    
11. Acetonitrile (Sigma-Aldrich, catalog number: 271004 )
    
12. 0.1% formic acid (Sigma-Aldrich, catalog number: 94318 )
    
13. p-coumaric acid (Sigma-Aldrich, catalog number: C9008 )
    
14. Ferulic acid (Sigma-Aldrich, catalog number: 128708 )
    
15. 50% methanol (Sigma-Aldrich, catalog number: 34860 )
    

Equipment

1. Mortar and pestle
   
2. Plastic spoon (appropriate for handling small volumes)
   
3. Shaking incubator, 37 °C
   
4. Centrifuge with a rotor that can accommodate 50 ml conical tubes and can reach 10,000 x g
   
5. Microcentrifuge
   
6. Vortex
   
7. Drying oven, 70 °C
   
8. Water bath, 65 °C
   
9. Chemical scoop (appropriate for handling small volumes)
   
10. Micropipette and tips (Mettler-Toledo, catalog number: L-STARTXLS+ )
    
11. Repeating pipettor with adaptor syringe for dispensing 200 µl (Mettler-Toledo, model: AR-E1 )
    
12. Speed Vac
    
13. Analytical balance
    
14. HPLC system with UV detector and autosampler (Shimadzu prominence modular HPLC: system controller CBM-20A, solvent delivery unit LC-20A, auto-sampler SIL-20A, column oven CTO-20A, UV-VIS detector SPD-20A)
    
15. HPLC glass vials (VWR, catalog number: 89220-128 )
    
16. Shim-pack XR-ODS column (column dimensions 3.0 x 75 mm, bead size 2.2 µm) (Shimadzu, model: Shim-pack XR-ODS )
    

Procedure