Salinity and Drought Treatment Assays in Kenaf (Hibiscus cannabinus L.)
洋麻的盐胁迫和干旱胁迫实验
发布: 2016年09月05日第6卷第17期 DOI: 10.21769/BioProtoc.1918 浏览次数: 9936
评审: Arsalan DaudiJian ChenMohan TC
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参见作者原研究论文
The authors used this protocol in:
Dec 2015
Abstract
Salinity and drought are the two main factors that cause fiber yield and quality losses in kenaf. It is reported that salinity and drought can affect more than 10% of arable land and cause a global decline in the average yields of major crops by more than 50%. Therefore, understanding plant tolerance of drought and salinity is of fundamental importance and has become the focus of intensive research. This protocol describes a simple and reproducible protocol to imitate natural salinity and drought stress under soil conditions. Even though the water-culture method is most frequently used for salinity and drought treatments, the soil-culture method in this study was more applicable to studying natural stress conditions.
Keywords: Salinity (盐度)
Drought (干旱)
Kenaf (Hibiscus cannabinus L.) (Kenaf（红麻）)
Materials and Reagents
- Sterile Petri dishes (JET, catalog number: TCD010090 )
- Sterile filter paper (Sigma-Aldrich, catalog number: Z241091 )
- Seeds: kenaf (cv Fuhong 992) [conserved in the Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops of Fujian Agriculture and Forestry University (China)].
- Sterile water (sterilized for 20 min at 121 °C/1 atm using an autoclave)
- Sterile soil (Fafard® growing mix 1-PV/C-1PV, Sun Gro Horticulture Canada Ltd.) (sterilized for 30 min at 121 °C/1 atm using an autoclave)
- Sodium hypochlorite (NaClO) solution (15%) (Sinopharm Chemical Reagent, catalog number: 7681-52-9 )
- Potassium nitrate (KNO3) (Sinopharm Chemical Reagent, catalog number: 7757-79-1 )
- Ammonium dihydric phosphate (NH4H2PO4) (Sinopharm Chemical Reagent, catalog number: 7722-76-1 )
- Ammonium nitrate (NH4NO3) (Sinopharm Chemical Reagent, catalog number: 6484-52-2 )
- Calcium nitrate [Ca(NO3)2·4H2O] (Sinopharm Chemical Reagent, catalog number: 13477-34-4 )
- Magnesium sulphate (MgSO4·7H2O) (Sinopharm Chemical Reagent, catalog number: 10034-99-8 )
- Ethylene diamine-N,N bis (2 hydroxyphenylacetic acid) Ferric sodium complex (Fe-EDDHA) (Sinopharm Chemical Reagent, catalog number: 16455-61-1 )
- Boric acid (H3BO3) (Sinopharm Chemical Reagent, catalog number: 10043-35-3 )
- Manganese chloride (MnCl2·4H2O) (Sinopharm Chemical Reagent, catalog number: 7773-01-5 )
- Zinc sulphate (ZnSO4·7H2O) (Sinopharm Chemical Reagent, catalog number: 7446-20-0 )
- Copper sulphate (CuSO4·5H2O) (Sinopharm Chemical Reagent, catalog number: 7758-98-7 )
- Sodium molybdate (Na2MoO4·2H2O) (Sinopharm Chemical Reagent, catalog number: 10102-40-6 )
- Sodium chloride (NaCl) (Sinopharm Chemical Reagent, catalog number: 7647-14-5 )
- PEG6000 (Sigma-Aldrich, catalog number: 81260 )
- 5% (v/v) sodium hypochlorite(see Recipes)
- Hoagland solution (see Recipes)
- 200 mM sodium chloride (NaCl) solution (see Recipes)
- 20% (w/v) PEG6000 solution (see Recipes)
Equipment
- Plastic pots (10 cm diameter and 8 cm depth)
- Clean bench (Suzhou AirTech , AirTech®, model: SW-CJ-1FD )
- Growth chamber (Yihengyiqi Shanghai, Blue pard®, model: MGC-300A )
- Autoclave (TOMY, model: SX-500 )
- Precision balance (± 0.001)
- Timer control
Procedure
文章信息
版权信息
© 2016 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Niu, X., Chen, M., Tao, A. and Qi, J. (2016). Salinity and Drought Treatment Assays in Kenaf (Hibiscus cannabinus L.). Bio-protocol 6(17): e1918. DOI: 10.21769/BioProtoc.1918.
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分类
植物科学 > 植物生理学 > 非生物胁迫
植物科学 > 植物生理学 > 植物生长
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