Salinity and Drought Treatment Assays in Kenaf (Hibiscus cannabinus L.)
洋麻的盐胁迫和干旱胁迫实验
发布: 2016年09月05日第6卷第17期 DOI: 10.21769/BioProtoc.1918 浏览次数: 10943
评审: Arsalan DaudiJian ChenMohan TC
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参见作者原研究论文
The authors used this protocol in:
Dec 2015
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Abstract
Salinity and drought are the two main factors that cause fiber yield and quality losses in kenaf. It is reported that salinity and drought can affect more than 10% of arable land and cause a global decline in the average yields of major crops by more than 50%. Therefore, understanding plant tolerance of drought and salinity is of fundamental importance and has become the focus of intensive research. This protocol describes a simple and reproducible protocol to imitate natural salinity and drought stress under soil conditions. Even though the water-culture method is most frequently used for salinity and drought treatments, the soil-culture method in this study was more applicable to studying natural stress conditions.
Keywords: Salinity (盐度)
Drought (干旱)
Kenaf (Hibiscus cannabinus L.) (Kenaf（红麻）)
Materials and Reagents
- Sterile Petri dishes (JET, catalog number: TCD010090 )
- Sterile filter paper (Sigma-Aldrich, catalog number: Z241091 )
- Seeds: kenaf (cv Fuhong 992) [conserved in the Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops of Fujian Agriculture and Forestry University (China)].
- Sterile water (sterilized for 20 min at 121 °C/1 atm using an autoclave)
- Sterile soil (Fafard® growing mix 1-PV/C-1PV, Sun Gro Horticulture Canada Ltd.) (sterilized for 30 min at 121 °C/1 atm using an autoclave)
- Sodium hypochlorite (NaClO) solution (15%) (Sinopharm Chemical Reagent, catalog number: 7681-52-9 )
- Potassium nitrate (KNO3) (Sinopharm Chemical Reagent, catalog number: 7757-79-1 )
- Ammonium dihydric phosphate (NH4H2PO4) (Sinopharm Chemical Reagent, catalog number: 7722-76-1 )
- Ammonium nitrate (NH4NO3) (Sinopharm Chemical Reagent, catalog number: 6484-52-2 )
- Calcium nitrate [Ca(NO3)2·4H2O] (Sinopharm Chemical Reagent, catalog number: 13477-34-4 )
- Magnesium sulphate (MgSO4·7H2O) (Sinopharm Chemical Reagent, catalog number: 10034-99-8 )
- Ethylene diamine-N,N bis (2 hydroxyphenylacetic acid) Ferric sodium complex (Fe-EDDHA) (Sinopharm Chemical Reagent, catalog number: 16455-61-1 )
- Boric acid (H3BO3) (Sinopharm Chemical Reagent, catalog number: 10043-35-3 )
- Manganese chloride (MnCl2·4H2O) (Sinopharm Chemical Reagent, catalog number: 7773-01-5 )
- Zinc sulphate (ZnSO4·7H2O) (Sinopharm Chemical Reagent, catalog number: 7446-20-0 )
- Copper sulphate (CuSO4·5H2O) (Sinopharm Chemical Reagent, catalog number: 7758-98-7 )
- Sodium molybdate (Na2MoO4·2H2O) (Sinopharm Chemical Reagent, catalog number: 10102-40-6 )
- Sodium chloride (NaCl) (Sinopharm Chemical Reagent, catalog number: 7647-14-5 )
- PEG6000 (Sigma-Aldrich, catalog number: 81260 )
- 5% (v/v) sodium hypochlorite(see Recipes)
- Hoagland solution (see Recipes)
- 200 mM sodium chloride (NaCl) solution (see Recipes)
- 20% (w/v) PEG6000 solution (see Recipes)
Equipment
- Plastic pots (10 cm diameter and 8 cm depth)
- Clean bench (Suzhou AirTech , AirTech®, model: SW-CJ-1FD )
- Growth chamber (Yihengyiqi Shanghai, Blue pard®, model: MGC-300A )
- Autoclave (TOMY, model: SX-500 )
- Precision balance (± 0.001)
- Timer control
Procedure
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文章信息
版权信息
© 2016 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Niu, X., Chen, M., Tao, A. and Qi, J. (2016). Salinity and Drought Treatment Assays in Kenaf (Hibiscus cannabinus L.). Bio-protocol 6(17): e1918. DOI: 10.21769/BioProtoc.1918.
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分类
植物科学 > 植物生理学 > 非生物胁迫
植物科学 > 植物生理学 > 植物生长
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