Simple Methods for Screening and Statistical Analysis of Leaf Epidermal Cells in Dicotyledonous Plants
双子叶植物中叶表皮细胞筛选和统计分析的简单方法
发布: 2016年09月05日第6卷第17期 DOI: 10.21769/BioProtoc.1916 浏览次数: 12654
评审: Marisa RosaJoëlle SchlapferAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Sep 2015
Abstract
Leaf epidermal cell size and number are positively correlated to leaf area. Stomata are specialized epidermal cells vital for gas exchange and water transpiration. So, observation and statistical analysis of the leaf epidermal cells are valuable for the study of leaf development and response to environmental stimulus. The classical method is using the scanning electron microscope (SEM), which is an expensive and time-consuming method, thus makes the large-scale screening of epidermis impractical. Here we provide simple but effective methods (agarose-based epidermal imprinting and tape-based epidermis tearing) for solving this problem without using the SEM.
Keywords: Epidermal cell (表皮细胞)
Stomata (stomata)
Epidermal imprinting (表皮印记)
Epidermis tearing (表皮撕裂)
ImageJ (ImageJ)
Materials and Reagents
- Glass slide (Leica, model: 3809299 )
- Scotch tape (1/2") (3M, Scotch®, model: 600 )
- Tobacco (one month old) and soybean seedlings (two weeks old)
- Distilled water
- Low melting point agarose (Thermo Fisher Scientific, InvitrogenTM, catalog number: 16520-050 )
- 3% agarose solution (see Recipes)
Equipment
- Thermostatic water bath (40 °C)
- Pointed tweezer (Tweezerman, model: 4091833 )
- Refrigerator (4 °C) (Haier, model: HT18TS77SP )
- Optical microscope (Olympus, model: BX51 )
- Objective used on microscope (Olympus, model: UPlanFI )
Software
- ImageJ (version 1.38x or above)
Procedure
文章信息
版权信息
© 2016 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
- Tao, J., Chen, S. and Zhang, J. (2016). Simple Methods for Screening and Statistical Analysis of Leaf Epidermal Cells in Dicotyledonous Plants. Bio-protocol 6(17): e1916. DOI: 10.21769/BioProtoc.1916.
- Tao, J. J., Cao, Y. R., Chen, H. W., Wei, W., Li, Q. T., Ma, B., Zhang, W. K., Chen, S. Y. and Zhang, J. S. (2015). Tobacco translationally controlled tumor protein interacts with ethylene receptor tobacco histidine kinase1 and enhances plant growth through promotion of cell proliferation. Plant Physiol 169(1): 96-114.
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分类
植物科学 > 植物细胞生物学 > 细胞结构
植物科学 > 植物生理学 > 组织分析
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