Materials and Reagents

1. Sterile cotton tipped applicators
2. Gauze pads (ATC Medical, Curity^TM, catalog number: 686132 )
3. Nolvasan surgical scrub (Zoetis Inc., catalog number: 8NOL413 )
4. Adult C57BL6/J laboratory mice (6 weeks old)
5. 70% ethanol
6. Isoflurane
7. Opthalmic ointment
8. 3.0% hydrogen peroxide solution
9. Sterile saline
10. VetBond glue (3M, catalog number: 1469SB )
11. 70% isopropanol

Equipment

1. Surgical Tools
   
   1. Scalpel handle (Fine Science Tools, catalog number: 10003-12 )
   2. Sterile scalpel blades (Fine Science Tools, catalog number: 10010-00 )
   3. Dumont forceps (Fine Science Tools, catalog number: 11251-10 )
   4. 5.0-mm diameter trephine (Figure 1) (Fine Science Tools, catalog number: 18004-50 )
   5. Pin vise (Figure 1) (ProEdge, catalog number: PRO58111 )
      
      
   
   Figure 1. Trephine in pin vise
   
   
2. Hot bead sterilizer (Fine Science Tools, catalog number: 18000-45 )
3. Warm water recirculator (Kent Scientific, catalog number: TP-700 )
4. Polystyrene weighing boat
5. Controlled cortical impact (CCI) device (Impact One Stereotaxic Impactor for CCI) (Leica Biosystems, catalog number: 39463920 )
   
   1. 3.0-mm diameter metal piston (Leica Biosystems , catalog number: 39463920)
6. Small animal stereotaxic instrument with mouse adapters (Kopf Instruments, model: 900 and 923-B )
7. Scale
8. Leather hole punch, sharp (Anytime Tools)
9. Incandescent lamp (50-75 watt)
10. Inclined plane device, custom made (Figure 2) (The angle board device is 50 x 60 cm in size and is covered with a vertically grooved rubber mat. The platform was attached to a hinge, allowing the researcher to manually adjust the angle of the board.)
    
    
    Figure 2. Inclined plane (custom made)
    

Procedure