Clonogenic Assay
克隆形成实验
发布: 2012年05月20日第2卷第10期 DOI: 10.21769/BioProtoc.187 浏览次数: 98041
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参见作者原研究论文
The authors used this protocol in:
Dec 2006
Abstract
Clonogenic assays serve as a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumor cells. A colony is defined as a cluster of at least 50 cells that can often only be determined microscopically. A clonogenic assay is the method of choice to determine cell reproductive death after treatment with ionizing radiation, but can also be used to determine the effectiveness of other cytotoxic agents. The following protocol has been modified from a published version (Franken et al., 2006).
Materials and Reagents
- Cell culture medium
- Phosphate buffered saline (PBS)
- Fetal bovine serum (FBS)
- Trypsin/ EDTA (Life Technologies, Invitrogen™, catalog number: 25200-056 )
- Crystal violet (Sigma-Aldrich, catalog number: C3886 )
- Methanol (Sigma-Aldrich, catalog number: 34860 )
- Glacial acetic acid (Sigma-Aldrich, catalog number: 320099 )
- Fixation solution
- Colony fixation solution (see Recipes)
- Crystal violet solution (see Recipes)
Equipment
- Cell culture petri dishes or six-well plates (Thermo Fisher Scientific, catalog number: 08-772-1B )
- Hemocytometer (Hausser Bright-Line) (Thermo Fisher Scientific, catalog number: 02-671-10 )
- Stereomicroscope (e.g., Nikon Eclipse, model: TS100 )
- Hemocytometer
- Incubator
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Yang, X. (2012). Clonogenic Assay. Bio-protocol 2(10): e187. DOI: 10.21769/BioProtoc.187.
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分类
癌症生物学 > 细胞死亡 > 细胞生物学试验
癌症生物学 > 通用技术 > 药物发现和分析
细胞生物学 > 细胞活力 > 细胞死亡
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