Materials and Reagents

1. Epithelial cell lines (e.g., Caco-2, IEC-6, CMT-93 cell lines)
   
2. DMEM medium (Thermo Fisher Scientific, Gibco^TM, catalog number: 11965-092 )
   
3. Fetal bovine serum (FBS) (Thermo Fisher Scientific, Gibco^TM, catalog number: 10082147 )
   
4. Collagen (Type I, Rat tail) (Corning, catalog number: 354236 )
   
5. L-cysteine (Sigma-Aldrich, catalog number: C7352 )
   
6. Lipopolysaccharide from E. coli 0111:B4 (Sigma-Aldrich, catalog number: L4391 )
   
7. Saline (Sigma-Aldrich, catalog number: S8776 )
   Note: It is also named as “Sodium chloride solution (0.9%)” on Sigma-Aldrich website.
   
8. Acetic acid (Sigma-Aldrich, catalog number: A6283 )
   
9. Penicillin-Streptomycin (Thermo Fisher Scientific, Gibco^TM, catalog number: 15140122 )
   
10. Collagen solution (see Recipes)
    
11. Cysteine solution (see Recipes)
    
12. Cell culture medium (see Recipes)
    

Equipment

1. ECIS Zθ (Theta) and 16 W array station (Applied BioPhysics, http://www.biophysics.com/products-ecisz0.php)
   
2. 8W10E+ Cultureware (Figure 1)
   
   
   Figure 1. 8W10E+ Cultureware (Applied BioPhysics, http://www.biophysics.com/cultureware.php#link8)
   

Software

1. ECIS Zθ Software

Procedure