Culture, Differentiation and Transfection of C2C12 Myoblasts
C2C12成肌细胞
发布: 2012年05月20日第2卷第10期 DOI: 10.21769/BioProtoc.172 浏览次数: 54702
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参见作者原研究论文
The authors used this protocol in:
Mar 2009
Abstract
C2C12 myoblasts are commonly used in biomedical laboratories as an in vitro system to study muscle development and differentiation. This protocol explains the basic procedures of culture, transfection and differentiation of C2C12 myoblast cells.
Materials and Reagents
- C2C12 myoblasts
- DMSO (Sigma-Aldrich, catalog number: 472301 )
- Fetal bovine serum (FBS)
- Horse serum
- DMEM (high glucose) (Life Technologies, Invitrogen™, catalog number: 11965142 )
- P/S solution
- Fugene HD (FHD) (Roche Diagnostics, catalog number: 04709691001 )
- Growth media (see Recipes)
- Transfection mix (see Recipes)
- Freezing media (see Recipes)
- Differentiation media (see Recipes)
Equipment
- Standard tabletop centrifuges
- Water bath
- CO2 incubator
- 100 mm culture dishes
- Eppendorf tube
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Jing, L. (2012). Culture, Differentiation and Transfection of C2C12 Myoblasts. Bio-protocol 2(10): e172. DOI: 10.21769/BioProtoc.172.
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分类
细胞生物学 > 细胞分离和培养 > 细胞分化
细胞生物学 > 细胞分离和培养 > 细胞生长
分子生物学 > DNA > 转染
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