通过测定叶绿素含量对瞬时表达后不同R基因/诱导子组合诱导产生的细胞坏死量进行定量测定

C. Jake Harris; David C. Baulcombe

doi:10.21769/BioProtoc.1670

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Peer-reviewed

Chlorophyll Content Assay to Quantify the Level of Necrosis Induced by Different R Gene/Elicitor Combinations after Transient Expression

通过测定叶绿素含量对瞬时表达后不同R基因/诱导子组合诱导产生的细胞坏死量进行定量测定

CH C. Jake Harris

DB David C. Baulcombe email

发布: 2015年12月05日第5卷第23期 DOI: 10.21769/BioProtoc.1670 浏览次数: 9430

评审: Feng LiAnonymous reviewer(s)

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The authors used this protocol in:

Cover of Proceedings of the National Academy of Sciences of the United States of America, featuring study using the protocol.

Dec 2013

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Abstract

This assay can be used to rapidly and accurately quantify levels of leaf necrosis induced after transient expression of R genes and elicitor combinations (Harris et al., 2013). It is based on the inverse correlation between level of necrosis and chlorophyll content in leaf tissue. It is adapted from the calculations described by (Strain et al., 1971).

Materials and Reagents

1.5 ml tube
Leaf discs
Note: Ensure that the leaf discs are fully submerged in the DMF solution.
N, N-Dimethylformamide (DMF) (Sigma-Aldrich, catalog number: D4551-250 ml )

Equipment

Spectrophotometer (Spectronic Biomate3) (Thermo Fisher Scientific, catalog number: 335904P )
Glass spectrophotometer cuvettes (Sigma-Aldrich, catalog number: Z276898 )
Note: Product Z276898 has been discontinued.

Procedure

English

中文翻译

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如何引用

Harris, C. J. and Baulcombe, D. C. (2015). Chlorophyll Content Assay to Quantify the Level of Necrosis Induced by Different R Gene/Elicitor Combinations after Transient Expression. Bio-protocol 5(23): e1670. DOI: 10.21769/BioProtoc.1670.