Permanent Occlusion of the Left Anterior Coronary Artery in the Rat
大鼠左前冠状动脉的永久性闭塞
发布: 2015年11月20日第5卷第22期 DOI: 10.21769/BioProtoc.1663 浏览次数: 8865
评审: Ivan ZanoniMartin V KolevAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Jan 2015
Abstract
Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological process is the main cause of cardiac evolution toward heart failure. Moreover, the outcome of patients after MI is largely dependent on the initial cardiac injury. Thus, this is of major clinical interest to develop new pharmacological strategies to limit infarct size and prevent or reverse left ventricular remodeling. Such preclinical cardiovascular treatments are often tested in rodents. The rat model of myocardial infarction is commonly used. In this model, the permanent ligation of the left anterior descending coronary artery is performed (Bousquenaud et al., 2013a).
After being used to this surgical technique and experimented, the operator will need 20 min per rat from the anesthesia to the rat recovering.
Materials and Reagents
- Sterile drapes
- Non-resorbable silk: Prolene 7.0 (Ethicon, catalog number: F1839 )
- Resorbable silk: Vicryl 4.0 (Ethicon, catalog number: V134 )
- Syringes (BD Biosciences, catalog number: 309659 )
- 2 shoelaces (around 20 cm)
- Tracheal tube (Fine Science Tools, catalog number: RSP-ETT1605 )
- Endotracheal tube (Kent Scientific Corporation, catalog number: RSP-ETT1605 )
- Rat Adult male Wistar rats weighing around 300 g (Charles River Laboratories International)
- Dermic Betadine 10%
- Antibiotics: Amoxicilline (Clamoxyl 100 mg/kg/24 h)
- Trichrome stain (Masson) Kit (Sigma-Aldrich, catalog number: HT15 )
- Anti-sarcomeric alpha actinin antibody [EA-53] (Abcam, catalog number: ab9465 )
Equipment
- Gaseous anesthesia delivery system (Minerve)
- Ventilator (Kent Scientific Corporation, model: TOPO220 )
- Fine curved forceps (Fine Science Tools, catalog number: 11272-30 )
- Shaver (Kent Scientific Corporation, catalog number: CL8787 )
- Rasor blade (Fine Science Tools, catalog number: 10008-13 )
- Scalpel (Fine Science Tools, catalog number: 10011-00 )
- Needle holders (Fine Science Tools, catalog number: 12001-13 )
- Retractor (Fine Science Tools, catalog number: 17012-11 )
Procedure
文章信息
版权信息
© 2015 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Bousquenaud, M., Marie, P. and Devaux, Y. (2015). Permanent Occlusion of the Left Anterior Coronary Artery in the Rat. Bio-protocol 5(22): e1663. DOI: 10.21769/BioProtoc.1663.
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分类
免疫学 > 动物模型 > 大鼠
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