Skin Wound Healing Model - Excisional Wounding and Assessment of Lesion Area
皮肤伤口愈合模型——切除性创伤和病变评估
发布: 2015年11月20日第5卷第22期 DOI: 10.21769/BioProtoc.1661 浏览次数: 23860
评审: Ivan ZanoniAchille BroggiMarco Di Gioia
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参见作者原研究论文
The authors used this protocol in:
Nov 2014
Abstract
This protocol focus on the most common surgical mouse model of cutaneous excisional wound healing used to study the cellular and molecular pathways involved in wound repair and regeneration as well as in translational applications such as the evaluation of new therapeutic modalities. This model allows the monitoring of the wound closure and the tissue collection for histological and molecular analyses. Briefly, full skin thickness excisional wounds are created on the dorsum of the mouse as the excision extends through the panniculosus carnosus. Wounds larger and minor diameters are then regularly measured and wound closure rate is calculated based on wound area relative to the original size.
Materials and Reagents
- 5 mm diameter circular biopsy punch (ABC, catalog number: 0418 )
- 10% Ketamine Hydrochloride (Agropecuária Tarumã, catalog number: 8565 )
- 2% Xylazine hydrochloride (Syntec)
- Potassium chloride (KCl) (Sigma-Aldrich, catalog number: P9541 )
- Potassium phosphate monobasic (KH2PO4) (Sigma-Aldrich, catalog number: P0662 )
- Sodium phosphate dibasic (Na2HPO4) (Sigma-Aldrich, catalog number: 255793 )
- Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S9888 )
- Saline solution (0.9% sodium chloride injectable solutio) (Equiplex)
- dH2O
- 70% alcohol (see Recipes)
- Phosphate buffered saline (PBS) (pH 7.2, 10x) (see Recipes)
- Phosphate buffered saline (PBS) (pH 7.2, 1x) (see Recipes)
Equipment
- Digital caliper (Mitutoyo, catalog number: 573-661 )
- Hair removal machine (Wahl and Toshico)
Procedure
文章信息
版权信息
© 2015 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Moreira, C. F., Cassini-Vieira, P., da Silva, M. F. and Barcelos, L. D. S. (2015). Skin Wound Healing Model - Excisional Wounding and Assessment of Lesion Area. Bio-protocol 5(22): e1661. DOI: 10.21769/BioProtoc.1661.
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分类
免疫学 > 动物模型 > 小鼠
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