Peer-reviewed
Alcian Blue – Alizarin Red Staining of Mouse Skeleton
阿尔辛蓝 - 茜素红对小鼠骨架染色
发布: 2012年04月20日第2卷第8期 DOI: 10.21769/BioProtoc.162 浏览次数: 32383
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Abstract
Our lab has used the Alcian blue – Alizarin red staining method with certain modifications to characterize skeleton deformities in mice lacking Pek/Perk, encoding a translational control eIF2alpha kinase.
Materials and Reagents
- Neural buffered formalin (Sigma-Aldrich, catalog number: HT5014 )
- Alcian blue 8GX (Sigma-Aldrich, catalog number: A5268 )
- Trypsin (Sigma-Aldrich, catalog number: T1426 )
- Saturated sodium borate (Sigma-Aldrich, catalog number: S9640 )
- Alizarin red (Sigma-Aldrich, catalog number: A3882 )
- Thymol (EM Life Science, catalog number: TX0615-1 )
- Ethanol
- Glacial acetic acid
- Potassium hydroxide
- Glycerol
- KOH
- Trypsin solution (see Recipes)
Equipment
- Conical tube
Procedure
English
中文翻译
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Zhang, P. (2012). Alcian Blue – Alizarin Red Staining of Mouse Skeleton. Bio-protocol 2(8): e162. DOI: 10.21769/BioProtoc.162.
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分类
发育生物学 > 形态建成
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