Chitin-challenged Mice Model to Study M2 Macrophages Polarization
基于几丁质刺激的小鼠模型研究M2巨噬细胞的极化
发布: 2015年09月05日第5卷第17期 DOI: 10.21769/BioProtoc.1584 浏览次数: 10113
评审: Ivan ZanoniSabine Le SauxAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Jan 2015
Abstract
Chitin is a key component of insects, fungi, and house-dust mites. Chitin has been shown to induce M2-type immune responses in vivo. Intranasal or intraperitoneal (i.p.) administration of chitin particles results in infiltration of eosinophils to the local sites and activation of macrophages with a M2 phenotype. Chitin-challenged mice model can be used to induce M2 macrophages polarization and thus to analyze the M2 phenotype from isolated peritoneal cells.
Keywords: Chitin (甲壳素)
Macrophages (巨噬细胞)
M2 polarization (M2极化)
Mice (小鼠)
Materials and Reagents
- Mice C57BL/6 male (age 8-12 weeks)
- Chitin (Sigma-Aldrich, catalog number: C9752 )
- 70% ethanol
- PBS (Lonza, catalog number: BE 17-515Q )
- 70 μm sterile cell strainers (BD Biosciences, catalog number: 352350 )
- RPMI 1640 (Lonza, catalog number: BE 12-115F )
- 10 ml syringe
- 21 G Needles
- 25 G Needles
- 15 ml conical tubes
Equipment
- Sonication bath, P-selecta ultrasons (150 W)
- Scissors
- Bench-top refrigerated centrifuge
Procedure
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文章信息
版权信息
© 2015 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Jiménez-García, L., Herránz, S., Luque, A. and Hortelano, S. (2015). Chitin-challenged Mice Model to Study M2 Macrophages Polarization. Bio-protocol 5(17): e1584. DOI: 10.21769/BioProtoc.1584.
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分类
免疫学 > 免疫细胞功能 > 巨噬细胞
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