Improve Research Reproducibility A Bio-protocol resource
Peer-reviewed
tooltip

Visualization of ex vivo Neutrophil Extracellular Traps by Fluorescence Microscopy

荧光显微镜法显示体外中性粒细胞胞外杀菌网

Koji Tanaka Koji Tanaka
TS Tadanobu Shimura
YT Yuji Toiyama
YO Yoshinaga Okugawa
YI Yasuhiro Inoue
TA Toshimitsu Araki
YM Yasuhiko Mohri
AM Akira Mizoguchi
MK Masato Kusunoki

发布: 2015年08月05日第5卷第15期 DOI: 10.21769/BioProtoc.1550 浏览次数: 12211

评审: Ivan ZanoniAchille BroggiMarco Di Gioia

download pdf PDF PDF
ask Q&A
引用
收藏
Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of PLOS ONE, featuring study using the protocol.

Nov 2014

Advertisement

人免疫细胞分选课程
Submit your protocol to the Special Issue on Technologies for RNA Detection
Presubmission Inquiry

实验方案合集

专注于特定技术或应用的、详细的、经过同行评审的实验方案合集

Cancer Research
Immunology
Developmental Biology
Microbiology
Neuroscience
Cell Imaging - A Special Collection for Cell Bio 2023
查看更多 more

相关实验方案

小鼠未成熟卵母细胞中非精子依赖性钙离子振荡的监测

小鼠未成熟卵母细胞中非精子依赖性钙离子振荡的监测

Sae Horiike [...] Hidehiko Ogawa

2026年02月05日 164 阅读

利用带网格培养皿实现活细胞延时成像到免疫荧光成像的衔接观察

利用带网格培养皿实现活细胞延时成像到免疫荧光成像的衔接观察

Nick Lang [...] Andrew D. Stephens

2026年02月20日 48 阅读

使用 Cell-APP 训练自定义细胞分割模型的方法

使用 Cell-APP 训练自定义细胞分割模型的方法

Anish J. Virdi and Ajit P. Joglekar

2026年02月20日 40 阅读

Abstract

Neutrophil extracellular traps (NETs) are extracellular DNAs decorated with nuclear and granular proteins such as histones, neutrophil elastase or myeloperoxidase. They exhibit fibrous mesh-like, web-like, or string-like structures. Here, we describe our protocol regarding visualization of ex vivo NETs released from neutrophils activated by lipopolysaccharide (LPS) using fluorescence microscopy.

Materials and Reagents

  1. Whole blood from wild-type C57/BL6 mice (Japan SLC, Inc.)
  2. Whole blood from human volunteers
  3. LPS (Escherichia coli, serotype 0111:B4) (Sigma-Aldrich, catalog number: L4391 )
  4. PolymorphprepTM (Axis Shield PoC AS, catalog number: 1114683 )
  5. RPMI 1640 medium (no phenol red) (Life Technologies, catalog number: 32404-014 )
  6. Fetal bovine serum (Life Technologies, catalog number: 12483-020 )
  7. ACK (Ammonium-chloride-potassium) lysing buffer (Lonza, catalog number: 10-548E )
  8. SYTOX Green (Life Technologies, InvitrogenTM, catalog number: S7020 )

Equipment

  1. Glass Pasteur pipets (Iwaki brand, Asahi Techno Glass Corporation)
  2. 96-well plates (TPP Techno Plastic Products AG)
  3. CO2 Incubator (SANYO)
  4. Fluorescence microscopy (Olympus, model: IX71 )

Procedure

English
中文翻译

文章信息

版权信息

© 2015 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Tanaka, K., Shimura, T., Toiyama, Y., Okugawa, Y., Inoue, Y., Araki, T., Mohri, Y., Mizoguchi, A. and Kusunoki, M. (2015). Visualization of ex vivo Neutrophil Extracellular Traps by Fluorescence Microscopy. Bio-protocol 5(15): e1550. DOI: 10.21769/BioProtoc.1550.

ris ris Download Citation in RIS Format

分类

免疫学 > 免疫细胞功能 > 嗜中性粒细胞

细胞生物学 > 细胞成像 > 荧光

细胞生物学 > 细胞成像 > 活细胞成像

您对这篇实验方法有问题吗？

在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。

post 发布问题
0 Q&A
pen 提交稿件