Improve Research Reproducibility A Bio-protocol resource
Peer-reviewed
tooltip

Visualization of ex vivo Neutrophil Extracellular Traps by Fluorescence Microscopy

荧光显微镜法显示体外中性粒细胞胞外杀菌网

Koji Tanaka Koji Tanaka
TS Tadanobu Shimura
YT Yuji Toiyama
YO Yoshinaga Okugawa
YI Yasuhiro Inoue
TA Toshimitsu Araki
YM Yasuhiko Mohri
AM Akira Mizoguchi
MK Masato Kusunoki

发布: 2015年08月05日第5卷第15期 DOI: 10.21769/BioProtoc.1550 浏览次数: 12175

评审: Ivan ZanoniAchille BroggiMarco Di Gioia

download pdf PDF PDF
ask Q&A
引用
收藏
Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of PLOS ONE, featuring study using the protocol.

Nov 2014

Advertisement

Submit your protocol to the Special Issue on Technologies for RNA Detection
人免疫细胞分选课程
Love Methods Week 2026: From Lab Notes to Lab Protocols
Presubmission Inquiry

实验方案合集

专注于特定技术或应用的、详细的、经过同行评审的实验方案合集

Cancer Research
Immunology
Developmental Biology
Microbiology
Neuroscience
Cell Imaging - A Special Collection for Cell Bio 2023
查看更多 more

相关实验方案

利用 RudLOV 研究细胞内物质运输

利用 RudLOV 研究细胞内物质运输

Tatsuya Tago [...] Akiko K. Satoh

2025年10月20日 1504 阅读

基于双SLIPT与双SLIPT-NVOC的脂质介导蛋白依序定位活细胞研究方法

基于双SLIPT与双SLIPT-NVOC的脂质介导蛋白依序定位活细胞研究方法

Kristina V. Bayer and Richard Wombacher

2025年11月05日 1584 阅读

使用 EdU 追踪脑部白细胞募集

使用 EdU 追踪脑部白细胞募集

Zoie K. Lipfert [...] David P. Sullivan

2025年12月05日 1541 阅读

Abstract

Neutrophil extracellular traps (NETs) are extracellular DNAs decorated with nuclear and granular proteins such as histones, neutrophil elastase or myeloperoxidase. They exhibit fibrous mesh-like, web-like, or string-like structures. Here, we describe our protocol regarding visualization of ex vivo NETs released from neutrophils activated by lipopolysaccharide (LPS) using fluorescence microscopy.

Materials and Reagents

  1. Whole blood from wild-type C57/BL6 mice (Japan SLC, Inc.)
  2. Whole blood from human volunteers
  3. LPS (Escherichia coli, serotype 0111:B4) (Sigma-Aldrich, catalog number: L4391 )
  4. PolymorphprepTM (Axis Shield PoC AS, catalog number: 1114683 )
  5. RPMI 1640 medium (no phenol red) (Life Technologies, catalog number: 32404-014 )
  6. Fetal bovine serum (Life Technologies, catalog number: 12483-020 )
  7. ACK (Ammonium-chloride-potassium) lysing buffer (Lonza, catalog number: 10-548E )
  8. SYTOX Green (Life Technologies, InvitrogenTM, catalog number: S7020 )

Equipment

  1. Glass Pasteur pipets (Iwaki brand, Asahi Techno Glass Corporation)
  2. 96-well plates (TPP Techno Plastic Products AG)
  3. CO2 Incubator (SANYO)
  4. Fluorescence microscopy (Olympus, model: IX71 )

Procedure

English
中文翻译

文章信息

版权信息

© 2015 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Tanaka, K., Shimura, T., Toiyama, Y., Okugawa, Y., Inoue, Y., Araki, T., Mohri, Y., Mizoguchi, A. and Kusunoki, M. (2015). Visualization of ex vivo Neutrophil Extracellular Traps by Fluorescence Microscopy. Bio-protocol 5(15): e1550. DOI: 10.21769/BioProtoc.1550.

ris ris Download Citation in RIS Format

分类

免疫学 > 免疫细胞功能 > 嗜中性粒细胞

细胞生物学 > 细胞成像 > 荧光

细胞生物学 > 细胞成像 > 活细胞成像

您对这篇实验方法有问题吗？

在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。

post 发布问题
0 Q&A
pen 提交稿件