Stereotaxic Infusion of LPS into Rat Substantia Nigra via Osmotic Minipump

Materials and Reagents

1. Eight-week-old male F344 rats, body weight 220–250 g
   
2. Nembutal
   
3. Carprofen
   
4. Betadine
   
5. 70% ethanol
   
6. Phosphate buffered saline (PBS)
   
7. 4% paraformaldehyde
   
8. Ocular lubricant (Puralube)
   
9. LPS (Escherichia coli 0111: B4) (Sigma-Aldrich)
   
10. 0.9% sterile normal saline or other vehicle for your reagents
    
11. LPS stock solution (see Recipes)
    

Equipment

1. Small-animal stereotaxic apparatus (rat stereotaxic apparatus)
   
2. Osmotic minipump (Alza Corporation)
   
3. Dental drill and #1 burrs
   
4. Dental cement
   
5. Stereotaxic frame
   
6. Cannula (stainless steel with cap)
   
7. Microknife
   
8. Scalpel (#10)
   
9. Tissue forceps
   
10. Gauze
    
11. Autoclips/suture materials
    

Procedure