Stereotaxic Infusion of LPS into Rat Substantia Nigra via Osmotic Minipump
通过微型渗透泵向大鼠脑黑质立体定向注射LPS
发布: 2012年04月20日第2卷第8期 DOI: 10.21769/BioProtoc.152 浏览次数: 12164
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参见作者原研究论文
The authors used this protocol in:
Jun 2002
Abstract
Stereotaxic infusion of LPS or your reagent of choice is an invaluable tool for the creation of chronic site-targeted lesions. Stereotaxic infusion of LPS has been used to establish chronic animal model of Parkinson’s disease, the most common neurodegenerative movement disorder. This protocol is especially useful to established chronic disease models and to study mechanisms of chronic central nervous system diseases.
Background
Materials and Reagents
- Eight-week-old male F344 rats, body weight 220–250 g
- Nembutal
- Carprofen
- Betadine
- 70% ethanol
- Phosphate buffered saline (PBS)
- 4% paraformaldehyde
- Ocular lubricant (Puralube)
- LPS (Escherichia coli 0111: B4) (Sigma-Aldrich)
- 0.9% sterile normal saline or other vehicle for your reagents
- LPS stock solution (see Recipes)
Equipment
- Small-animal stereotaxic apparatus (rat stereotaxic apparatus)
- Osmotic minipump (Alza Corporation)
- Dental drill and #1 burrs
- Dental cement
- Stereotaxic frame
- Cannula (stainless steel with cap)
- Microknife
- Scalpel (#10)
- Tissue forceps
- Gauze
- Autoclips/suture materials
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Gao, H. (2012). Stereotaxic Infusion of LPS into Rat Substantia Nigra via Osmotic Minipump. Bio-protocol 2(8): e152. DOI: 10.21769/BioProtoc.152.
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分类
神经科学 > 神经系统疾病
细胞生物学 > 组织分析 > 组织分离
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