Materials and Reagents

1. Fungal blast (Magnaporthe oryzae) stocks (NIAS Genbank, http://www.gene.affrc.go.jp/index_en.php)
   
2. Oat meal agar plates (9 cm diameter) (Difco oatmeal agar; Difco)
   
3. Rice (Oryza sativa subsp. japonica) seeds
   
4. Soil (Bonsol No.2; Sumitomo Chemical Co., Ltd. http://www.sumitomo-chem.co.jp/)
   
5. Sterilized water
   

Equipment

1. Bacteria spreader
   
2. Conical plastic tubes (50 ml) (Greiner)
   
3. Eppendorf tubes (1.5 ml) (Eppendorf)
   
4. Filter paper (Advantec)
   
5. Fungal growth cabinet with black fluorescent lights (Toshiba, model: FL15BLB )
   
6. Glass funnel
   
7. Hemocytometer (Fuchs-Rosenthal, Hirschmann EM Techcolor)
   
8. KimWipe (Nippon Paper Crecia Co., Ltd., model: Wiper S-200 )
   
9. Microscope (Leica Microsystems)
   
10. Petri dishes (9 cm diameter)
    
11. Sprayer (Figure 1C; Tokyo Koyama Plastic, Co., Ltd., model: K380 )
    
12. Sterilized painting brush (Figure 1B)
    
13. Dew chamber (Ozawa corporation, model: 513A )
    

Procedure