Plant Materials and Growth Conditions of Japanese Morning Glory (Ipomoea nil cv. Violet)
日本牵牛花培养条件的建立及植物材料的分析
发布: 2015年05月20日第5卷第10期 DOI: 10.21769/BioProtoc.1478 浏览次数: 10674
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参见作者原研究论文
The authors used this protocol in:
Sep 2014
Abstract
Japanese morning glory (Ipomoea nil) is a summer annual vine that typically produces ephemeral flowers. This plant has been used extensively to investigate flowers, including studies on flowering, flower color, and petal senescence. Here we describe the materials and optimal growth conditions used to grow the Japanese morning glory cultivar, “Violet”, in a growth chamber. Violet plants are transformable with Agrobacterium (Rhizobium), and we have used this cultivar to examine petal senescence (Shibuya et al., 2009; Shibuya et al., 2014).
Keywords: Morning glory (牵牛花)
Ipomoea (甘薯)
Flower (花)
Plant (植物)
Materials and Reagents
- Japanese morning glory cultivar, “Violet” (Ipomoea nil cv. Violet) seeds
Note: Seeds of the Japanese morning glory cultivar, “Violet” (Ipomoea nil cv. Violet), as well as other cultivars can be obtained through the National BioResource Project, Japan (http://www.shigen.nig.ac.jp/asagao/).
- Horticultural soil (Nippi Horticulture soil No. 1; Nihon Hiryo)
- Fertilizer (Hyponex, catalog number: 15-30-15 )
Equipment
- Growth chamber
- 12 cm-diameter pots
- Metal wire (1 m)
- Utility knife
Procedure
文章信息
版权信息
© 2015 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Shibuya, K. (2015). Plant Materials and Growth Conditions of Japanese Morning Glory (Ipomoea nil cv. Violet). Bio-protocol 5(10): e1478. DOI: 10.21769/BioProtoc.1478.
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分类
植物科学 > 植物生理学 > 植物生长
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