Materials and Reagents

1. Vigna mungo MYMIV-resistant recombinant inbred line, VMR84
   
2. mirPremier microRNA isolation kit (Sigma-Aldrich, catalog number: SNC10 )
   
3. Mir-X miRNA FirstStrand synthesis and SYBR qRT-PCR kit (Takara Bio Company, Clontech, catalog number: 638314 )
   
4. 2-mercaptoethanol (Sisco Research Laboratories, catalog number: 1324196 )
   
5. Ethanol (Merck, catalog number: K41540783 )
   
6. Liquid nitrogen and dry ice
   
7. RNase free water (Bangalore Genei, catalog number: 612151181001730 )
   
8. Agarose (Sisco Research Laboratories, catalog number: 0140229 )
   
9. Ethidium bromide (Sisco Research Laboratories, catalog number: 054817 )
   
10. 1x TAE buffer (see Recipes)
    
11. Ethidium bromide stock (see Recipes)
    

Equipment

1. Thermocycler (DNA Engine Cycler, model: PTC-200 )
   
2. Real-time qPCR (Biorad iQ5 Real-Time PCR Detection System)
   
3. Centrifuge (Thermo Fisher Scientific, model: MicroCL21 )
   
4. Electrophoresis apparatus (Bangalore Genei)
   
5. Heat block or water bath
   
6. Mortar and pestle
   
7. NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific)
   

Procedure