Materials and Reagents

1. Young anthers (at the microspore stage of lily buds normally from 30 to 65 mm of bud length)
   
2. Lithium chloride (LiCl) (Merck KGaA, catalog number: 105679 )
   
3. Tris-base (United States Biological, catalog number: 75825 )
   
4. Ethylenediaminetetraacetic acid (EDTA) (United States Biological, catalog number: 15699 )
   
5. Sodium dodecyl sulfate (SDS) (AppliChem, catalog number: A2263 )
   
6. Isoamylalcohol (J.T. Baker^®, catalog number: JT9038-1 )
   
7. Phenol (pH 4.0) (United States Biological, catalog number: 77510 )
   
8. Chloroform (Merck KGaA, catalog number: 102445 )
   
9. Deionized distilled water (ddH₂O)
   
10. Diethylpyrocarbonate (DEPC) (Sigma-Aldrich, catalog number: D5758 )
    
11. Sodium acetate (NaOAc) (Sigma-Aldrich, catalog number: S2889 )
    
12. Isopropanol (Merck KGaA, catalog number: 107022 )
    
13. 75% ethanol (analytical grade)
    
14. Liquid nitrogen
    
15. 0.1% DEPC ddH₂O (RNase-free water) (see Recipes)
    
16. Extraction buffer (see Recipes)
    
17. 3 M sodium acetate (see Recipes)
    
18. 10 mM sodium acetate buffer (see Recipes)
    
19. 4 M LiCl (see Recipes)
    
20. 75% ethanol (see Recipes)
    

Equipment

1. Scalpel
   
2. Tweezers (stainless, length: 11.5 cm; tip: 0.1 mm)
   
3. Ceramic mortar and pestle
   
4. 1.5 ml Eppendorf
   
5. Centrifuge (Eppendorf, model: 5424 )
   
6. Fume hood
   
7. Speed vacuum (Savant Systems LLC, model: SC110 )
   

Procedure