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Hanging Drop Aggregation Assay of Breast Cancer Cells

乳腺癌细胞的悬滴聚集试验

Yong Teng Yong Teng

发布: 2015年02月05日第5卷第3期 DOI: 10.21769/BioProtoc.1393 浏览次数: 21704

评审: HongLok LungAnonymous reviewer(s)

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Abstract

Hanging drop assay can be used to investigate cell-cell cohesion and cell-substratum adhesion through generation of 3D spheroids under physiological conditions. It also can be used to elucidate the role of cell-cell or cell-ECM interactions in specifying spatial relationships between two (or more) different cell populations. This simple method requires no specialized equipment and provides a means of generating tissue-like cellular aggregates for measurement of biomechanical properties for molecular and biochemical analysis in a physiologically relevant model.

Keywords: Hanging drop (悬滴) search Cancer (癌症) search Aggregation (聚集) search Migration (迁移) search

Materials and Reagents

  1. Human breast cancer cell lines T-47D (ATCC, catalog number: ATCC® HTB-133TM ), MCF7 (ATCC, catalog number: ATCC® HTB-22TM )
  2. Trypsin-EDTA (0.05%) (phenol red) (Life Technologies, catalog number: 25300062 )
  3. Gibco® RPMI media 1640 (Life Technologies, catalog number: 11875-085 )
  4. Gibco® PBS (Life Technologies, catalog number: 10010023 )
  5. Fetal bovine serum (Life Technologies, catalog number: 16000-044 )
  6. Gibco® penicillin-streptomycin-glutamine (100x) (Life Technologies, catalog number: 10378-016 )
  7. Complete medium (see Recipes)

Equipment

  1. CorningTM culture dishes (Corning, catalog number: 430591 )
  2. Inverted microscope (ZEISS)
  3. Haemocytometer (Thermo Fisher Scientific, catalog number: 02-671-52 )

Procedure

English
中文翻译

文章信息

版权信息

© 2015 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Teng, Y. (2015). Hanging Drop Aggregation Assay of Breast Cancer Cells. Bio-protocol 5(3): e1393. DOI: 10.21769/BioProtoc.1393.

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分类

癌症生物学 > 侵袭和转移 > 细胞生物学试验 > 细胞粘附

癌症生物学 > 通用技术 > 肿瘤形成 > 细胞粘附

细胞生物学 > 细胞运动 > 细胞迁移

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