真菌病原体灰葡萄孢菌对番茄和苹果果实的感染分析

Zhanquan Zhang; Guozheng Qin; Boqiang Li; Shiping Tian

doi:10.21769/BioProtoc.1311

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Peer-reviewed

Infection Assays of Tomato and Apple Fruit by the Fungal Pathogen Botrytis cinerea

真菌病原体灰葡萄孢菌对番茄和苹果果实的感染分析

ZZ Zhanquan Zhang

GQ Guozheng Qin

BL Boqiang Li

Shiping Tian email

发布: 2014年12月05日第4卷第23期 DOI: 10.21769/BioProtoc.1311 浏览次数: 12979

评审: Zhaohui LiuAnonymous reviewer(s)

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The authors used this protocol in:

Cover of Molecular Plant Microbe Interactions, featuring study using the protocol.

Jun 2014

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Abstract

Botrytis cinerea (B. cinerea) is an aggressive fungal pathogen that infects more than 200 plant species. Furthermore, the pathogen can attack fruits of some plants, such as tomato and apple. B. cinerea has become one of the model systems in molecular phytopathology because of its economic importance and sophisticated genetic operation methods. Virulence assays are very important in the study of fungal pathogenesis. This protocol details the artificial inoculation procedure of B. cinerea on tomato and apple fruits. It also can be used to analyse the virulence of postharvest fungal pathogens on other fruits, such as pear, peach, jujube and so on.

Materials and Reagents

Freshly harvested tomatoes and apples (fully ripe)
Botrytis cinerea (B05.10): Supplied by Prof. Paul Tudzynski Westfaelische Wilhelms-Universitaet Muenster, Germany
KH₂PO₄ (Beijing Chemical Works, catalog number: HG/T 1274-1993 )
Glucose (Beijing Chemical Works, catalog number: HG/T 3475-1999 )
2% sodium hypochlorite (Xilong Chemical, catalog number: 7681-52-9 )
Potato
Dextrose
PDA medium (see Recipes)
2% sodium hypochlorite (see Recipes)
KH₂PO₄-glucose solution (see Recipes)

Equipment

Glass stick
Hemacytometer (QIUJING, model: XB-K-25 )
Cheesecloth
Vortexer (Select BioProducts, model: SBS100-2 )
Optical microscope (Chongqing Optec Instrument, model: B203LED )
Clean bench (Donglian Electronic & Technology Development, model: SCB-1520 )
Pipette
Plastic basket
Sterile nail (approximately 3 mm in diameter)

Procedure

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Zhang, Z., Qin, G., Li, B. and Tian, S. (2014). Infection Assays of Tomato and Apple Fruit by the Fungal Pathogen Botrytis cinerea. Bio-protocol 4(23): e1311. DOI: 10.21769/BioProtoc.1311.