Materials and Reagents

1. Freshly harvested tomatoes and apples (fully ripe)
   
2. Botrytis cinerea (B05.10): Supplied by Prof. Paul Tudzynski Westfaelische Wilhelms-Universitaet Muenster, Germany
   
3. KH₂PO₄ (Beijing Chemical Works, catalog number: HG/T 1274-1993 )
   
4. Glucose (Beijing Chemical Works, catalog number: HG/T 3475-1999 )
   
5. 2% sodium hypochlorite (Xilong Chemical, catalog number: 7681-52-9 )
   
6. Potato
   
7. Dextrose
   
8. PDA medium (see Recipes)
   
9. 2% sodium hypochlorite (see Recipes)
   
10. KH₂PO₄-glucose solution (see Recipes)
    

Equipment

1. Glass stick
   
2. Hemacytometer (QIUJING, model: XB-K-25 )
   
3. Cheesecloth
   
4. Vortexer (Select BioProducts, model: SBS100-2 )
   
5. Optical microscope (Chongqing Optec Instrument, model: B203LED )
   
6. Clean bench (Donglian Electronic & Technology Development, model: SCB-1520 )
   
7. Pipette
   
8. Plastic basket
   
9. Sterile nail (approximately 3 mm in diameter)
   

Procedure