Materials and Reagents

1. Arabidopsis seeds (Col-0 and tt mutants)
   
2. 6 M HCl
   
3. Sterile distilled water
   
4. Dye solution (for coloration and reaction with PAs) containing 1% w/v vanillin (Sangon Biotech, catalog number: VT0974- 100g) and 6 M HCl stored in brown bottle
   Note: The dye solution should be used after preparation as soon as possible (not longer than half an hour).
   
5. Vanillin reagent (see Recipes)
   

Equipment

1. Brown bottle (or glass bottle covered with aluminum foil)
   
2. 1.5 ml microcentrifuge tubes
   
3. Glass slides (25.4 mm x 76.2 mm) and coverslips (20 mm x 20 mm)
   
4. Dissecting needle and tweezer
   
5. An SZ61-zoom stereomicroscope (OLYMPUS, model: SZ61)
   
6. A compound light microscope (OLYMPUS, model: BX61 )
   

Procedure