Induction of Tigecycline Resistance in Acinetobacter baumannii
鲍氏不动杆菌中替加环素抗性的诱导
发布: 2014年12月05日第4卷第23期 DOI: 10.21769/BioProtoc.1307 浏览次数: 8496
评审: Arsalan DaudiKanika GeraAnonymous reviewer(s)
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The authors used this protocol in:
May 2014
Abstract
Multidrug resistance Acinetobacter baumannii (A. baumannii) (MDRAB) has emerged as a serious threat in hospitals in recent years. Currently, there are few antibiotics, including tigecycline, available to treat infections caused by MDRAB effectively. Both tigecycline-resistant and tigecycline-susceptible isogenic strains of MDRAB are valuable in understanding the mechanisms underlying tigecycline resistance. To get the isogenic strains in the laboratory, we describe a protocol for induction of tigecycline resistance in A. buamannii by serial passage to plates with tigecycline of different concentrations. The minimal inhibitory concentration of A. baumannii by tigecycline was determined according to the protocol “Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii” (Lin et al., 2014b).
Keywords: Acinetobacter baumannii (鲍氏不动杆菌)
Tigecycline (替加环素)
Drug resistance (耐药性)
Serial passages (串行通道)
Materials and Reagents
- A. baumannii (ATCC, catalog number: 17978 )
- Tigecycline (Wyeth, catalog number. 0220620-09-7 )
- Tryptone (Pronadisa, catalog number: 1612 )
- Yeast extract (Pronadisa, catalog number: 1702 )
- NaCl (MDBio, catalog number: 101-1647-14-5 )
- 99% glycerol (Honeywell International, Riedel-deHaen, catalog number: 5523 )
- Lysogeny broth (LB) (see Recipes)
- 20% glycerol (see Recipes)
Equipment
- 50 ml polystyrene culture tubes (sterile)
- 37 °C shaking and static incubators
- Multichannel pipette (volume ranges 10 μl-1,000 μl)
Procedure
文章信息
版权信息
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Lin, M., Lin, Y. and Lan, C. (2014). Induction of Tigecycline Resistance in Acinetobacter baumannii. Bio-protocol 4(23): e1307. DOI: 10.21769/BioProtoc.1307.
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分类
微生物学 > 抗微生物试验 > 抗细菌试验
细胞生物学 > 细胞分离和培养 > 细胞生长
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