稳定Caco-2细胞系的建立

Improve Research Reproducibility A Bio-protocol resource

Peer-reviewed

Stable Caco-2 Cell Line Construction

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Abstract

This protocol describes the construction of Caco-2 stable cell lines using the Lipofectamine transfection method.

Materials and Reagents

Caco-2 cell
Lipofectamine TM transfection reagent. (Life Technologies, Invitrogen™, catalog number: 18324-020 )
Dulbecco’s Modification of Eagles Medium (DMEM) (Life Technologies, Invitrogen™)
Trypsin-EDTA (Life Technologies, Invitrogen™/Gibco^®)
Opti-MEM (Life Technologies, Invitrogen™/Gibco^®)
2x FBS (Life Technologies, Invitrogen™/Gibco^®)
G418 (Life Technologies, Invitrogen™)
Trypsin (Life Technologies, Invitrogen™/Gibco^®)
Cloning ring, vacuum grease (all autoclaved)
Trypsin