Analysis of Intestinal Permeability in Mice
小鼠肠道通透性分析
发布: 2014年11月20日第4卷第22期 DOI: 10.21769/BioProtoc.1289 浏览次数: 48257
评审: Kate HannanIsabel Cristiane da SilvaAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Apr 2014
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Abstract
The intestinal epithelial layer serves as a barrier against pathogens and ingested toxins, which are present in the lumen of the intestine. The importance of the intestinal epithelial barrier is emphasized by the alterations in paracellular permeability and tight junction functions observed in inflammatory bowel disease (IBD) and colon cancer.
Keywords: epithelial barrier (上皮屏障)
inflammation (炎症)
tumor (肿瘤)
FITC-dextran (FITC-葡聚糖)
Materials and Reagents
- 8-10-week old mice (in-house bred mice mostly C57BL/6 background; both male and female)
- Fluorescein isothiocyanate conjugated dextran (FITC-dextran) (Sigma-Aldrich, catalog number: FD4 )
- Sterile 1x phosphate-buffered saline (PBS) (pH 7.4)
- Anesthesia (isoflurane) (ESTEVE, catalog number: 103287025 )
Equipment
- BD Microtainer SST tubes (BD Biosciences, catalog number: 365968 )
- 1 ml syringes (ENFA, catalog number: JS1 )
- Autoclaved oral gavage needles (22 Gauge /25 mm long) (Fine Science Tools, catalog number: 18061-22 )
- Spectrophotofluorometer (BioTek Instruments, catalog number: FLx800 )
- 96-well microplates (flat bottom) (Corning, catalog number: 3650 )
- Dissection equipment (forceps, tweezers, scissors)
- Balance
- Table top anesthesia machine (Parkland Scientific, catalog number: V3000PK )
Procedure
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文章信息
版权信息
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Gupta, J. and Nebreda, A. R. (2014). Analysis of Intestinal Permeability in Mice. Bio-protocol 4(22): e1289. DOI: 10.21769/BioProtoc.1289.
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分类
细胞生物学 > 组织分析 > 组织染色
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