Materials and Reagents

1. Barley seedlings
   
2. Radioisotopes ³²P or ³³P labeled NaH₂PO₄ dissolved in water (MP Biomedicals, PerkinElmer or American Radiolabeled Chemicals)
   
3. 5 mM CaSO₄ solution
   
4. Hydroponic culture solution (see Recipes)
   

Equipment

1. Cling film
   
2. 1.5 ml plastic tubes
   
3. 15 ml plastic tubes (1.5 ml tube is fitted by opening a hole in the lid) (Figure 1)
   
4. Cotton
   
5. Plastic sponge
   
6. Imaging plate (FCR Imaging Plate for general purpose) (Fujifilm Corporation) and plate cassette (FCR standard cassette) (Fujifilm Corporation)
7. Imaging analyzer (GE Healthcare, model: Typhoon 9400 or other Radioisotope imaging analyzers)
   

Procedure