Extraction of Ions from Leaf Sections
叶片切片的离子提取
发布: 2014年07月05日第4卷第13期 DOI: 10.21769/BioProtoc.1174 浏览次数: 7143
评审: Ru Zhang
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参见作者原研究论文
The authors used this protocol in:
Oct 2013
Abstract
The concentration of ions in plant cells and tissues is an important factor to determine their functions and conditions. Here, we describe the method to extract ions from leaf sections for measurements with an ion chromatogram. This method is available for not only barley but also other plant species.
Keywords: Ion chromatography (离子色谱法)
Barley (大麦)
Inorganic ion (无机离子)
Materials and Reagents
- Barley seedlings
- Milli-Q water
- Hydroponic culture solution (see Recipes)
Equipment
- 1.5 ml and 2 ml plastic tubes
- Scissors and forceps
- Sample crusher (Kurabo Industries, model: SH-48 )
- 7 mm zirconia beads (Kurabo Industries, model: Z-07 )
- Vortex mixer
- Dry thermo unit (Taitec, catalog number: DTU-18 )
- 0.45 µm filters (EMD Millipore, catalog number: SLLHH04NL )
- 1.0 ml syringe
- Centrifuge
- Ion chromatogram (Thermo Fisher Scientific, Dionex, model: ICS-1500 ) equipped with an ion exchange column (AS-12 for anions and CS-16 for cations)
Procedure
文章信息
版权信息
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Kurita, Y., Kanno, S., Ohnishi, M. and Mimura, T. (2014). Extraction of Ions from Leaf Sections. Bio-protocol 4(13): e1174. DOI: 10.21769/BioProtoc.1174.
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分类
植物科学 > 植物生理学 > 离子分析
生物化学 > 其它化合物 > 离子
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