Extraction of Ions from Leaf Sections

Materials and Reagents

1. Barley seedlings
   
2. Milli-Q water
   
3. Hydroponic culture solution (see Recipes)
   

Equipment

1. 1.5 ml and 2 ml plastic tubes
   
2. Scissors and forceps
   
3. Sample crusher (Kurabo Industries, model: SH-48 )
   
4. 7 mm zirconia beads (Kurabo Industries, model: Z-07 )
   
5. Vortex mixer
   
6. Dry thermo unit (Taitec, catalog number: DTU-18 )
   
7. 0.45 µm filters (EMD Millipore, catalog number: SLLHH04NL )
   
8. 1.0 ml syringe
   
9. Centrifuge
   
10. Ion chromatogram (Thermo Fisher Scientific, Dionex, model: ICS-1500 ) equipped with an ion exchange column (AS-12 for anions and CS-16 for cations)
    

Procedure