Materials and Reagents

1. Dissected rosette leaves from Arabidopsis
   
2. Petals from the well-opened flower
   
3. Ethanol (Sigma-Aldrich, Catalog number: 459844 )
   
4. Chloral hydrate (Sigma-Aldrich, Catalog number: 15307 )
   
5. Glycerol (Sigma-Aldrich, Catalog number: G7757 )
   
6. Washing solution (see Recipes)
   
7. Clearing solution (see Recipes)
   

Equipment

1. Stereomicroscope (Zeiss, model: Zeiss Stemi 2000 )
   
2. Digital Cameral (Canon, model: Canon powershot S5IS )
   
3. Glass slides and thin coverslips
   
4. Differential interference contrast (DIC) microscope (Nikon Corporation, model: ECLIPSE 80i) with CCD camera (Nikon Corporation, model: DS-Ri1 )
   

Software

1. Image J software (http://rsbweb.nih.gov/ij/index.html).
   
2. Cell Counter plugin (http://rsbweb.nih.gov/ij/plugins/cell-counter.html)
   

Procedure