屏障功能试验

Tia  DiTommaso; Floris  Foijer

doi:10.21769/BioProtoc.1133

Improve Research Reproducibility A Bio-protocol resource

提交稿件
订阅
登录
/
注册
- 个人主页
- 编辑个人信息
- 修改密码
- 退出
CN
- EN - English
- CN - 中文

Peer-reviewed

Barrier Function Assay

屏障功能试验

TD Tia DiTommaso

Floris Foijer email

发布: 2014年05月20日第4卷第10期 DOI: 10.21769/BioProtoc.1133 浏览次数: 11273

评审: Lin FangFanglian HeAnonymous reviewer(s)

PDF

Q&A

引用

Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of Proceedings of the National Academy of Sciences of the United States of America, featuring study using the protocol.

Feb 2013

Special Issue on Protocols for Organoid Research

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

相关实验方案

脑组织微血管的分离与成像

Josephine K. Buff [...] Sophia M. Shi

2025年08月05日 3082 阅读

小鼠视交叉上核的组织形态计量学分析

Sophia A.M.B. Villanueva [...] Cristina A. Ghiani

2026年06月05日 312 阅读

类器官免疫染色的组织学处理方法

Lisa Brossard [...] Maxime M. Mahe

2026年07月20日 419 阅读

Abstract

This protocol serves to determine the integrity of the barrier function of (murine) epidermis. Defects in the barrier function lead to dehydration and infection in neonatal mice/humans. One possible way to assess epidermal barrier integrity is by a dye penetration assay as described hereunder. This assay should be done on unfixed, untreated tissues (e.g. formaldehyde- or glutaraldehyde-fixed) or on whole mouse embryos (E18.5). This protocol was adapted from Hardman (1998).

Keywords: Skin integrity (皮肤的完整性)