Karyotype Analysis
染色体组型分析
发布: 2014年05月20日第4卷第10期 DOI: 10.21769/BioProtoc.1129 浏览次数: 29682
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参见作者原研究论文
The authors used this protocol in:
Jan 2013
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Abstract
A chromosome is the structure that organizes DNA and protein in cells. It is a single piece of coiled DNA containing coding and non-coding sequences. Human cells have 23 pairs of chromosomes including 22 pairs of autosomes and one pair of sex chromosome, giving a total of 46 per cell. In tumor cells, chromosomal instability has been considered to be one of the hallmarks of tumor formation. Here we use the karyotype analysis to separate the chromosomes and observe the chromosomes in tumor cells with a microscope.
Chromosomal instability (染色体不稳定性)
Tumor formation (肿瘤的形成)
Materials and Reagents
- Cell lines: FaDu (ATCC, catalog number: HTB-43TM )
- Trypsine (Life Technologies, Gibco®, catalog number: 15400-054 )
- 10% fetal bovine serum (Thermo Fisher Scientific, catalog number: SH30071.03 )
- 1% Penicillin-Streptomycin (10,000 U/ml; 10,000 μg/ml) (Life Technologies, Gibco®, catalog number: 15140-122 )
- Colcemid (10 μg/ml) (Life Technologies, Gibco®, catalog number: 15212-012 )
- Hypotonic solution (0.075 M KCl) (J.T.Baker®, catalog number: 7447-40-7 )
- Glacial acetic acid (J.T.Baker®, catalog number: 64-19-7 )
- Fixative (see Recipes)
- Growth medium (see Recipes)
Equipment
- 10 cm cell culture dishes (Trueline Valve Corpotation, catalog number: TR4002 )
- Centrifuge tube
- Microscope (OLYMPUS, model: DP71 )
- CO2 incubator
- Centrifuge at room temperature (using “relative centrifugal force, rcf”)
- Water bath
Procedure
文章信息
版权信息
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Chou, C. and Yang, M. (2014). Karyotype Analysis. Bio-protocol 4(10): e1129. DOI: 10.21769/BioProtoc.1129.
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分类
癌症生物学 > 基因组不稳定性及突变 > 细胞生物学试验 > DNA结构和改变
细胞生物学 > 细胞成像 > 固定细胞成像
细胞生物学 > 细胞结构 > 染色体
