Materials and Reagents

1. Yeast strains
   Note: BG14 was used as the C. glabrata parental strain. The hst1Δ and the cta1Δ null mutants were used as a positive and the negative controls, respectively.
   
2. Catalase from bovine liver (Sigma-Aldrich, catalog number: C9322 )
   
3. Bradford reagent (Sigma-Aldrich, catalog number: B6916 )
   
4. Bovine serum albumin - fraction V (Sigma-Aldrich, catalog number: 85040C )
   
5. Zirconia/silica beads (0.5 mm diameter) (Bio Spec Products, catalog number: 11079105z )
   
6. Sterile water
   
7. Ice
   
8. One tablet of protease inhibitors cOmplete ULTRA Mini EASYpack is used in 10 ml of phosphate buffer (Roche Diagnostics, catalog number: 05 892 970 001 )
   
9. H₂O₂ (Sigma-Aldrich, catalog number: 349887 )
   
10. Catalase lyophilized powder (Sigma-Aldrich, catalog number: C9322)
    
11. YPD broth (see Recipes)
    
12. 50 mM Phosphate buffer (PB) (pH 7.0) (see Recipes)
    
13. 30 mM H₂O₂ (see Recipes)
    
14. Catalase solution (see Recipes)
    

Equipment

1. Orbital incubator shaker
   
2. Microfuge tubes
   
3. 50 ml conical tubes
   
4. Corning 96 well clear flat bottom (Corning, catalog number: 3595 )
   
5. Standard 10 mm light path quartz cuvette with PTFE cover
   
6. UV/Vis Spectrophotometer (Shimadzu, model: UV-1700 , catalog number: 206-55401-92)
   
7. Microplate spectrophotometer system (Benchmark Plus Microplate reader) (Bio-Rad Laboratories, catalog number: 170-6931 )
   
8. Centrifuge (Beckman Coulter, model: Allegra^® 25R, catalog number: 369464 )
   
9. Microfuge
   
10. Stopwatch
    
11. Parafilm
    

Procedure