Materials and Reagents

1. Plant (e.g., 2-3 true leaf stage)
   
2. Newly-emerged (e.g., 2-5 d old) female whiteflies
   

Equipment

1. Ice-bag or box
   
2. Glass dish (Diameter = 6 cm)
   
3. Gold wire (Diameter = 12.5 μm, http://www.epgsystems.eu/products.php)
   
4. Silver glue (Vial of 2ml glue, http://www.epgsystems.eu/products.php)
   
5. Faraday cage (self-made cage with10 mesh wire net, length = 60 cm, width = 60 cm, height = 70 cm)
   
6. Insect-proof cage (self-made cage with 80 mesh nylon screening, length = 40 cm, width = 40 cm, height = 60 cm)
   
7. Transparent tubes (self-made tube with bottle, 3 cm long, inner diameter = 5 mm)
   
8. Small ice-chilled glass dish
   
9. Giga-8 DC-EPG system with 10⁹ Ohm input resistance (Wageningen University, http://www.epgsystems.eu/products.php)
   
10. DI710-UL analogue-to-digital converter (DATAQ Instruments, http://www.epgsystems.eu/products.php)
    
11. Computer
    
12. Dissecting microscope
    

Software

1. PROBE 3.4 software (Wageningen University, advanced version can be downloaded from http://www.epgsystems.eu/downloads.php)

Procedure