Materials and Reagents

1. Potato (S. tuberosum cv. Désirée) tissue culture plantlets
   
2. 70% ethanol
   
3. Bleach (6% sodium hypochlorite)
   
4. Tween 20
   
5. Acetosyringone (Fisher Scientific, catalog number: AC11554 )
   
6. Timentin (PhytoTechnology Laboratories^®, catalog number: T869 )
   
7. Selective antibiotics
   
8. Mannitol (Fisher Scientific, catalog number: M120 )
   
9. Glycine
   
10. Nicotinic acid (Sigma-Aldrich, catalog number: N0761 )
    
11. Pyridoxine HCl (Sigma-Aldrich, catalog number: P6280 )
    
12. Thiamine HCl (Sigma-Aldrich, catalog number: T1270 )
    
13. Folic acid (Fisher Scientific, catalog number: BP2519 )
    
14. Biotin (Fisher Scientific, catalog number: BP232 )
    
15. MS salt (Caisson Laboratories, catalog number: MSP01-50LT )
    
16. Inositol (Fisher Scientific, catalog number: AC122261000 )
    
17. Sucrose
    
18. 6-benzylamino purine (BAP) (Sigma-Aldrich, catalog number: B3408 )
    
19. 1-naphthalene-acetic acid (NAA) (Sigma-Aldrich, catalog number: N0640 )
    
20. 3-indoleacetic acid (IAA) (Sigma-Aldrich, catalog number: I2886 )
    
21. Trans-zeatin-riboside (Sigma-Aldrich, catalog number: Z0876 )
    
22. YM medium (see Recipes)
    
23. MSVI vitamins (see Recipes)
    
24. JHMS vitamins (see Recipes)
    
25. 3R vitamins (see Recipes)
    
26. CIM medium (see Recipes)
    
27. 3C5ZR plates with selective agent (see Recipes)
    
28. Propagation medium (see Recipes)
    
29. TPS buffer (see Recipes)
    

Equipment

1. Sterile magenta boxes (sterilized by autoclaving)
   
2. Sterile petri dishes (Fisher scientific, catalog number: FB0875712 )
   
3. Sterile forceps and scalpel (sterilized by heat treatment using a Bunsen burner)
   
4. Sterile glass tubes with caps (sterilized by autoclaving) (tubes, Fisher Scientific, catalog number: 14-961-34 ; caps, Fisher Scientific, catalog number: 14-957-91D )
   
5. Sterile inoculating loop (Fisher Scientific, catalog number: 22-363-604 )
   
6. Tissue grinders
   
7. Glass culture tubes (sterilized by autoclaving)
   
8. 3M Micropore tape (Fisher Scientific, catalog number: 1530-0 and 1530-1 )
   
9. Agrobacterium glycerol stock
   
10. 50 ml conical centrifuge tubes
    
11. Tissue culture biosafety cabinet
    
12. Incubator shaker (New Brunswick Scientific, model: C24KC )
    
13. Centrifuge
    
14. S. tuberosum growth chambers (we use several types of growth chambers such as I-66LLVL from Percival)
    
15. Hot block or water bath
    
16. PCR thermal cycler (Bio-Rad Laboratories, model: C1000 touch)
    

Procedure