ImmunoFISH for Mice and Baboons Frozen Sections
小鼠和狒狒冰冻切片的ImmunoFISH试验
发布: 2013年12月20日第3卷第24期 DOI: 10.21769/BioProtoc.1000 浏览次数: 9712
评审: Lin Fang
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参见作者原研究论文
The authors used this protocol in:
Apr 2012
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Abstract
This protocol is optimized for immunoFISH staining of OCT section of mouse tissues. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) (Le et al., 2010) and FISH against telomeric DNA.
Keywords: ImmunoFISH (immunoFISH)
DNA damage response (DNA损伤反应)
Telomeres (端粒酶)
Baboon tissues (Baboon组织)
Materials and Reagents
- Tissue
- OCT
- 4% formaldehyde
- PBS
- Triton
- Goat serum
- BSA
- Primary antibody: 53BP1 #NB 100-304 rabbit from Novus
- Second antibody: goat anti-rabbit Alexa Fluor® 488 Dye
- Triton
- Glycine
- Mowiol 4-88 reagent (Calbiochem®)
- Formamide
- Tris HCl, pH 7.4
- Telomeric PNA probe (TelC-Cy3 from PANAGENE, catalog number: F1002-5 )
- Tween-20
- DAPI
- Hybridization mixture (see Recipes)
- Blocking reagent (Roche Diagnostics, catalog number: 11096176001 ) (see Recipes)
- Wash solution I (see Recipes)
- Wash solution II (see Recipes)
Equipment
- Glass slide
- Metal thermoblock
- Humidified chamber
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Rossiello, F., Fumagalli, M. and di Fagagna, F. D. (2013). ImmunoFISH for Mice and Baboons Frozen Sections. Bio-protocol 3(24): e1000. DOI: 10.21769/BioProtoc.1000.
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分类
细胞生物学 > 细胞成像 > 荧光
细胞生物学 > 组织分析 > 组织分离
生物化学 > 蛋白质 > 免疫检测 > 免疫染色法
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