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Request a Protocol

If you would like a more detailed, step-by-step version of a protocol derived from a research article, use the Request a Protocol (RaP) feature, powered by Bio-protocol.

As part of our efforts to make science more reproducible, Bio-protocol has collaborated with partners such as Science/AAAS and eLife to make it easier to view and request detailed protocols directly from their published articles.

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“AAAS launches collaboration with Bio-protocol, in support of scientific reproducibility”
AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE

NEWS RELEASE 13-AUG-2019

eLife Latest: Request detailed protocols easily via Bio-protocol integration
Our new reproducibility-focused integration with Bio-protocol lets you request a detailed protocol from any article on elife.

Inside eLife. 21-Aug-2019

Fill out the request form, and we will contact the author(s) on your behalf. If the author agrees to share the protocol details you are requesting, we will let you know via email. Please do not request that authors share details that are already available in their research article or supplementary materials.

Research Article

Synthesis of EPA- and DHA-Enriched Structured Acylglycerols at the sn-2 Position Starting from Commercial Salmon Oil by Enzymatic Lipase Catalysis under Supercritical Conditions
Gretel Dovale-Rosabal et al.

DOI: 10.3390/molecules26113094

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4.1. DRCSO Characterization

4.2. DRCSO Fatty Acid Composition

4.3. Preparation of EPA/DHA sAG by Enzymatic Intra–Interesterification Process under CO2-Supercritical Conditions (CO2SC) Using Different Extraction Times

4.4. Purification of EPA/DHA sAG by Neutralization with NaOH

4.5. Fatty Acid Composition and Quantification of Fractionated EPA/DHA sAG

4.6. Identification of EPA/DHA sAG and DRCSO by TLC

4.7. Positional Analysis of EPA and DHA by Mass Spectrometry (MALDI-TOF)

4.8. Statistical Analysis

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