Request a Protocol - Bio-protocol

Request a Protocol

If you would like a more detailed, step-by-step version of a protocol derived from a research article, use the Request a Protocol (RaP) feature, powered by Bio-protocol.

As part of our efforts to make science more reproducible, Bio-protocol has collaborated with partners such as Science/AAAS and eLife to make it easier to view and request detailed protocols directly from their published articles.

Click HERE to learn more about the RaP feature.

“AAAS launches collaboration with Bio-protocol, in support of scientific reproducibility”
AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE

NEWS RELEASE 13-AUG-2019

eLife Latest: Request detailed protocols easily via Bio-protocol integration
Our new reproducibility-focused integration with Bio-protocol lets you request a detailed protocol from any article on elife.

Inside eLife. 21-Aug-2019

Fill out the request form, and we will contact the author(s) on your behalf. If the author agrees to share the protocol details you are requesting, we will let you know via email. Please do not request that authors share details that are already available in their research article or supplementary materials.

Research Article

Synthesis, LC-MS/MS analysis, and biological evaluation of two vaccine candidates against ticks based on the antigenic P0 peptide from R. sanguineus linked to the p64K carrier protein from Neisseria meningitidis
Luis Javier González et al.

DOI: 10.1007/s00216-021-03569-0

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Choose one protocol to request:

Peptide synthesis

Synthesis of pP0-p64K conjugates

SDS-PAGE analysis of conjugates

Proteolytic digestion of conjugates

LC-MS/MS analyses

Identification of conjugation sites based on the detection of type 2 peptides

Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) analysis of the conjugates

Immunization and challenge experiments in rabbits

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